规格 | 价格 | 库存 | 数量 |
---|---|---|---|
5mg | ¥1363.00 | 现货 | |
10mg | ¥2181.00 | 现货 | |
25mg | ¥4363.00 | 现货 |
特色产品
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
产品描述
Oligomycin A是ATP合成酶的抑制剂,其抑制作用发生于依赖ATP和氧化磷酸化的线粒体耦合膜过程[1]。
Oligomycin A阻断ATP合酶的质子通道,这是ADP通过氧化磷酸化转化为ATP的必要步骤,从而抑制ATP合酶。Oligomycin A对ATP合成的抑制过程可以显著减少电子传递链的电子流动。
来自美国国家癌症研究所的对60种人癌细胞系的研究表明,oligomycin是37000个分子中前0.1%的最具选择性的细胞毒性药物。在HeLa癌细胞系中,H+ -ATP-synthase的抑制剂 oligomycin(5 mg/ml)强烈抑制细胞呼吸,这种抑制作用是与ATP合成相关的。在一系列癌细胞中,100 ng/ml oligomycin在1小时内可完全抑制癌细胞的氧化磷酸化活性,在6小时内可诱导不同程度的糖酵解。作为H + -ATP- synthase F0部分的抑制剂,oligomycin可以抑制TNF诱导的细胞凋亡。使用不同浓度的oligomycin和rotenone减少了高达94%的耗氧量,表明线粒体在这个过程中的重要作用。使用oligomycin可完全消除H2O2的增加[2-4]。
参考文献:?
[1]. Jastroch M, Divakaruni AS, Mookerjee S, et al. Mitochondrial proton and electron leaks. Essays Biochemistry, 2010, 47:53-67.
[2]. Shchepina LA, Pletjushkina OY, Avetisyan AV, et al. Oligomycin, inhibitor of the F-0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. Oncogene, 2002, 53: 8149-8157.
[3]. Salomon AR, Voehringer DW, Herzenberg LA, et al. Understanding and exploiting the mechanistic basis for selectivity of polyketide inhibitors of F0F1-ATPase. Proceedings of The National Academy of Sciences of The United States of America, 2000, 97(26): 14766-14771.
[4]. Alexander R, Adina V, Ivan B, et al. Overcoming intrinsic multi-drug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1Bhigh? cells. Cancer Cell, 2013, 23(6): 811-825
产品性质
物理外观 | A solid |
CAS号 | 579-13-5 |
分子式 | C45H74O11 |
分子量 | 791.06 |
化学名称 | 4-ethyl-11,12,15,19-tetrahydroxy-6'-(2-hydroxypropyl)-5',10,12,14,16,18,20,26,29-nonamethylspiro[24,28-dioxabicyclo[23.3.1]nonacosa-5,7,21-triene-27,2'-oxane]-13,17,23-trione |
溶解度 | insoluble in H2O; ≥17.43 mg/mL in EtOH; ≥9.89 mg/mL in DMSO |
SMILES | CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C |
存储条件 | -20°C |
运输条件 | 蓝冰 |
SDF文件 | Download SDF |
生物相关数据
质量控制
操作说明
产品引用文献
- 1. Elias Adriaenssens, Thanh Ngoc Nguyen, et al. "Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD." bioRxiv. September 25, 2023.
- 2. Shumeng Hao, Sulin Zhang, et al. "Goliath induces inflammation in obese mice by linking fatty acid β‐oxidation to glycolysis." EMBO Rep. 2023 Apr 5;24(4):e56932. PMID: 36862324
- 3. Jing Lv, Ying Yi, et al. "Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cells." Cell Death Discov. 2022 Feb 17;8(1):69. PMID: 35177589
- 4. Xingyuan Zhai, Kai Liu, et al. "Mitochondrial C1qbp promotes differentiation of effector CD8+T cells via metabolic-epigenetic reprogramming." Sci Adv. 2021 Dec 3;7(49):eabk0490. PMID: 34860557
- 5. Tian C, Yuan Z, et al. "Inhibition of glycolysis by a novel EGFR/HER2 inhibitor KU004 suppresses the growth of HER2+cancer." Exp Cell Res. 2017 May 19. pii: S0014-4827(17)30297-5. PMID: 28532652