Ferrostatin-1 (Fer-1)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ferrostatin-1 是一种选择性的ferroptosis抑制剂。
Ferroptosis是一种受调控的、氧化的、非凋亡性细胞死亡,已发现Ferrostatin-1是其强效的抑制剂。通过阻断胱氨酸转运和谷胱甘肽产生,Fer-1可以抑制氧化的、铁-依赖性的癌细胞死亡。据报道,Fer-1通过抑制脂质过氧化防止亨廷顿氏病细胞模型的死亡。[ 1 ]
参考文献:
1. Skouta, R., et al., Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J Am Chem Soc, 2014. 136(12): p. 4551-6.
- 1. Qiang Wang, et al. "Verteporfin induces lipid peroxidation and ferroptosis in pancreatic cancer cells." Free Radic Biol Med. 2024 Feb 20:212:493-504. PMID: 38184120
- 2. Zhijing He, He Zhou, et al. "A bimetallic dual-targeting nanoplatform for combinational ferroptosis activation/epigenetic regulation/photothermal therapy against breast cancer and tumor microenvironment remodeling." Chemical Engineering Journal Volume 479, 1 January 2024, 147466.
- 3. Siyu Zhang, Huihan Yu, et al. "Copper homeostasis based on Cuproptosis-related signature optimizes molecular subtyping and treatment of glioma." Research Square. 28 Dec, 2023.
- 4. Mingchao Mu, Qin Zhang, et al. "3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis." Cancer Gene Ther. 2023 Aug 9. PMID: 37558749
- 5. Yu Zhang, Xiyou Du, et al. "A Vanadium-Based Nanoplatform Synergizing Ferroptotic-like Therapy with Glucose Metabolism Intervention for Enhanced Cancer Cell Death and Antitumor Immunity." ACS Nano. 2023 Jun 5. PMID: 37272777
- 6. Fan Zhang, Lixiang Wu, et al. "FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling." CNS Neurosci Ther. 2023 Nov;29(11):3351-3363. PMID: 37211949
- 7. Shibin Ding, Jinjin Jiang, et al. "Ambient particulate matter exposure plus chronic ethanol ingestion exacerbates hepatic fibrosis by triggering the mitochondrial ROS-ferroptosis signaling pathway in mice." Ecotoxicol Environ Saf. 2023 May:256:114897. PMID: 37043943
- 8. Qingyu Zhang, Ning Li, et al. "ACSL1-induced ferroptosis and platinum resistance in ovarian cancer by increasing FSP1 N-myristylation and stability." Cell Death Discov. 2023 Mar 8;9(1):83. PMID: 36882396
- 9. Dong S, Zheng L, et al. "Loss of Lactate/Proton Monocarboxylate Transporter 4 Induces Ferroptosis via the AMPK/ACC Pathway and Inhibition of Autophagy on Human Bladder Cancer 5637 Cell Line" J Oncol 2023;2023 PMID: 36718218
- 10. Kempen CG, Deragon MA, et al. "Necroptosis of Lung Epithelial Cells Triggered by Ricin Toxin and Bystander Inflammation." Cell Physiol Biochem 2023 Jan 25;57(1) PMID: 36695077
- 11. Jiawen Cui, Qin Zhou, Chintada Nageswara Rao, et al. "4-tert-butylphenol triggers common carp hepatocytes ferroptosis via oxidative stress, iron overload, SLC7A11/GSH/GPX4 axis, and ATF4/HSPA5/GPX4 axis." Ecotoxicol Environ Saf. 2022 Sep 1;242:113944. PMID: 35926411
- 12. Li Cai, Xiaoqing Hu, et al. "Long non-coding RNA ADAMTS9-AS1 attenuates ferroptosis by Targeting microRNA-587/solute carrier family 7 member 11 axis in epithelial ovarian cancer." Bioengineered. 2022 Apr;13(4):8226-8239. PMID: 35311457
- 13. Tingting Liao, Xia Xu, et al. "DJ-1 upregulates the Nrf2/GPX4 signal pathway to inhibit trophoblast ferroptosis in the pathogenesis of preeclampsia." Sci Rep. 2022 Feb 21;12(1):2934. PMID: 35190654
- 14. QING SHI, RUI LIU, et al. "Ferroptosis inhibitor ferrostatin‑1 alleviates homocysteine‑induced ovarian granulosa cell injury by regulating TET activity and DNA methylation." Mol Med Rep. 2022 Apr;25(4):130. PMID: 35169856
- 15. Xinxin Ren, Xiang Wang, et al. "Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation." Aging (Albany NY). 2022 Jan 25;14(2):961-974. PMID: 35077390
- 16. Amjad Ali, Jasmin Shafarin, et al. "Co-targeting BET bromodomain BRD4 and RAC1 suppresses growth, stemness and tumorigenesis by disrupting the c-MYC-G9a-FTH1axis and downregulating HDAC1 in molecular subtypes of breast cancer." Int J Biol Sci. 2021 Oct 25;17(15):4474-4492. PMID: 34803511
- 17. Zongbo Wei, Caili Hao, et al. "Aging lens epithelium is susceptible to ferroptosis." Free Radic Biol Med. 2021 May 1;167:94-108. PMID: 33722625
- 18. Junting Xiao, Shanshan Zhang, et al. "Arsenite induces ferroptosis in the neuronal cells via activation of ferritinophagy." Food Chem Toxicol. 2021 May;151:112114. PMID: 33722599
- 19. Yue Yang, yuanyuan Lu, et al. "Identification of A New Kind of Phenazine Compounds Attenuating The Stemness of Breast Cancer Cells Through Triggering Ferroptosis." Research Square. March 1st, 2021.
- 20. Binghua Liu, Weiyan Wang, et al. "Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells." Cell Death Dis. 2021 Mar 3;12(3):230. PMID: 33658488
- 21. Zhuo Sun, Yidan Cao, et al. "Antiangiogenic effect of arsenic trioxide in HUVECs by FoxO3a‐regulated autophagy." J Biochem Mol Toxicol. 2021 Feb 16;e22728. PMID: 33592126
- 22. Xinzhi Yang, Jiangang Liu, et al. "miR-18a promotes glioblastoma development by down-regulating ALOXE3-mediated ferroptotic and anti-migration activities." Oncogenesis. 2021 Feb 12;10(2):15. PMID: 33579899
- 23. Ali Ghoochani, En-Chi Hsu, et al. "Ferroptosis inducers are a novel therapeutic approach for advanced prostate cancer." Cancer Res. 2021 Mar 15;81(6):1583-1594. PMID: 33483372
- 24. Qi Wang, Yaxun Guo, et al. "RNA binding protein DAZAP1 promotes HCC progression and regulates ferroptosis by interacting with SLC7A11 mRNA." Exp Cell Res. 2021 Feb 1;399(1):112453. PMID: 33358859
- 25. Xia Qin, Qianghu Tang, et al. "Zinc Oxide Nanoparticles Induce Ferroptotic Neuronal Cell Death in vitro and in vivo." Int J Nanomedicine. 2020 Jul 27;15:5299-5315. PMID: 32884256
- 26. Tianshu Wu, Xue Liang, et al. "Induction of ferroptosis in response to graphene quantum dots through mitochondrial oxidative stress in microglia." Particle and Fibre Toxicology volume 17, Article number: 30 (2020).11 July 2020.
- 27. Meng P, Zhang S, et al. "Arsenite induces testicular oxidative stress in vivo and in vitro leading to ferroptosis." Ecotoxicol Environ Saf. 2020;194:110360. PMID: 32151864
- 28. Hu Z, Zhang H, et al. "VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis." Cell Death Dis. 2020;11(1):73. Published 2020 Jan 29. PMID: 31996668
- 29. Zhou HH, Chen X, et al. "Erastin Reverses ABCB1-Mediated Docetaxel Resistance in Ovarian Cancer." Front Oncol, 9, 1398 2019 Dec PMID: 31921655
- 30. Xiaohong Zhang, Xueshuang Xing, et al. "Ionizing radiation induces ferroptosis in granulocyte-macrophage hematopoietic progenitor cells of murine bone marrow." Int J Radiat Biol , 1-35 2020 Jan 6. PMID: 31906761
- 31. Wang H, Liu C, et al. "Inhibition of LONP1 protects against erastin-induced ferroptosis in Pancreatic ductal adenocarcinoma PANC1 cells." Biochem Biophys Res Commun. 2019 Dec 7. pii: S0006-291X(19)32315-0. PMID: 31822343
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 262.35 |
Cas No. | 347174-05-4 |
Formula | C15H22N2O2 |
Solubility | ≥149 mg/mL in DMSO; ≥99.6 mg/mL in EtOH with ultrasonic; insoluble in H2O |
SDF | Download SDF |
Canonical SMILES | NC1=C(NC2CCCCC2)C=CC(C(OCC)=O)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
健康中等多刺神经元,少突胶质细胞,肾近端小管细胞 |
溶解方法 |
该化合物在DMSO中的溶解度大于9.8 mg/mL。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。 |
反应条件 |
10 nM,100 nM,1 μM |
应用 |
Fer-1(10 nM、100 nM和1 μM)显著增加健康MSN的数量。Fer-1(1 μM)增加健康MSN的数量。Fer-1(100 nM)保护少突胶质细胞免于胱氨酸剥夺。Fer-1(0.1-2 μM)可防止羟基喹啉和硫酸亚铁铵引起的致死性,HQ和Fe使用量为各10 μM。 |
注意事项 |
由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。 |
References: [1]. Skouta R, Dixon S J, Wang J, et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models[J]. Journal of the American Chemical Society, 2014, 136(12): 4551-4556. |
Description | Ferrostatin-1(Fer-1)是一种选择性的erastin诱导的ferroptosis抑制剂,EC50值为60 nM。 | |||||
靶点 | erastin induced ferroptosis | |||||
IC50 | 60 nM (EC50) |
质量控制和MSDS
- 批次:
化学结构
![Ferrostatin-1 (Fer-1)](http://www.apexbt.com//media/diy/images/struct/A4371.png)
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