Cy5 TSA Fluorescence System Kit
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Fanchu Zeng, Zhijin Fan, et al. "Tumor Microenvironment Activated Photoacoustic-Fluorescence Bimodal Nanoprobe for Precise Chemo-immunotherapy and Immune Response Tracing of Glioblastoma." ACS Nano. 2023 Oct 24;17(20):19753-19766. PMID: 37812513
- 2. Jie Hong, Jie Liu, et al. "MiR-3180 inhibits hepatocellular carcinoma growth and metastasis by targeting lipid synthesis and uptake." Cancer Cell Int. 2023 Apr 11;23(1):66. PMID: 37041584
- 3. Horwitz, Lorraine. "Novel Sensory Pathways for Thermosensation and Pain Under Physiological and Pathological Conditions." University of Michigan Library. 2022.
- 4. Huayue Li, Zhongwu Chen, et al. "MiR-4310 regulates hepatocellular carcinoma growth and metastasis through lipid synthesis." Cancer Lett. 2021 Oct 28;519:161-171. PMID: 34303763
APExBIO公司的酪胺信号放大(TSA)系统可用于检测荧光免疫细胞化学(ICC)、免疫组织化学(IHC)和原位杂交(ISH)技术中的低丰度靶点,可将信号灵敏度提高100倍。
TSA荧光试剂盒使用辣根过氧化物酶(HRP)直接催化固定化酶周围的荧光基团共价沉积。该标记过程迅速(少于10分钟), 沉积标记可直接在标准或共聚焦显微镜下观察。 TSA荧光试剂盒也可以与抗荧光组合使用。
TSA技术可用于酶缀合物和合适的生色底物的明场显微镜检查。与普通实验相比,使用TSA试剂可显著提高信号灵敏度,同时保持稳定的特异性和分辨率。此外,TSA试剂可以显着减少一抗或探针的消耗量。本试剂盒(K1052)荧光标记信号为Cyanine 5,可在激发和发射波长(648nm/667nm)下显微检测信号。
酪胺信号放大系统原理简图
使用常规方法用一抗和二抗标记细胞或组织样品。与二抗缀合的辣根过氧化物酶催化标记的酪酰胺转化为反应性基团, 之后酪胺基团与附近的酪氨酸残基共价结合,提供高密度标记。
Complete Kit | 100-300 slides | 200-600 slides |
1X Amplification Diluent | 30 mL | 60 mL |
Cyanine 5 Tyramide (dry, dissolve in 60 μL DMSO) | 1 tube | 2 tubes |
Blocking Reagent | 6 g | 12 g |
Store Cyanine 5 Tyramide in the dark at -20°C for 1 year. Keep 1X Amplification Diluent and Blocking Reagent at 4°C for 1 year. |