Catalog No. A8627
Bafilomycin A1（别名：巴佛洛霉素A1）
V-ATPase抑制剂
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 500ug | ￥1787.00 | 现货 | |
| 1mg | ￥3033.00 | 现货 | |
| 5mg | ￥11537.00 | 现货 |
CAS号：88899-55-2纯度：99.43%
仅用于科研，不用于诊疗。未经明确授权不得转售。
A
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特色产品
Annexin V-FITC/PI Apoptosis Kit
用于双染检测细胞凋亡和坏死
- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
Phosbind Acrylamide
分离和检测磷酸化/非磷酸化蛋白
- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
Cell Counting Kit-8 (CCK-8)
用于细胞增殖和细胞毒性试验
- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
Cy5 TSA Fluorescence System Kit
Cy5荧光标记的酪胺信号放大系统
- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)
用于观察mRNA运送，定位，翻译
- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
HotStart™ 2X SYBR Green qPCR Master Mix
用于荧光定量目标DNA或cDNA
- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus
高效的Cy5修饰RNA转录试剂盒
- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Bafilomycin A1是一种选择性的空泡H+ ATPases（V-ATPases）抑制剂，I50值为4-400 nmol/mg[1]。
Bafilomycin A1作用于不同类型的膜ATPases，作用于真菌（粗糙脉孢菌）、植物（Z. Mays）和动物（牛肾上腺髓质）的空泡ATPases，I50值分别为400 nmol/mg、4 nmol/mg 和50 nmol/mg。此外，bafilomycin A1对空泡ATPases的抑制活性通过检测其对H+泵活性的影响而进一步确定。H+的易位被10 nM bafilomycin A1完全抑制[1]。其它的研究表明，bafilomycin A1强烈抑制破骨细胞的坑形成活性。通过免疫电镜观察bafilomycin A1处理或非处理破骨细胞中V-ATPase的亚细胞定位。这些结果表明，bafilomycin A1破坏V-ATPase在皱褶缘的定位，导致牙质表面酸化的缺乏[2]。注：I50值是指抑制每mg蛋白中50%的ATPase活性所需要的bafilomycin A1的umol量。
参考文献：
1. Bowman E J, Siebers A, Altendorf K. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells[J]. Proceedings of the National Academy of Sciences, 1988, 85(21): 7972-7976.
2. Takami M, Suda K, Sahara T, et al. Involvement of vacuolar H+-ATPase in incorporation of risedronate into osteoclasts[J]. Bone, 2003, 32(4): 341-349.
产品性质
| 物理外观 | A crystalline solid |
| CAS号 | 88899-55-2 |
| 分子式 | C35H58O9 |
| 分子量 | 622.84 |
| 化学名称 | (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-16-[(2S,3R,4S)-4-[(2R,4R,5S,6R)-2,4-dihydroxy-5-methyl-6-propan-2-yloxan-2-yl]-3-hydroxypentan-2-yl]-8-hydroxy-3,15-dimethoxy-5,7,9,11-tetramethyl-1-oxacyclohexadeca-3,5,11,13-tetraen-2-one |
| 溶解度 | Soluble in DMSO |
| SMILES | CC1CC(=CC=CC(C(OC(=O)C(=CC(=CC(C1O)C)C)OC)C(C)C(C(C)C2(CC(C(C(O2)C(C)C)C)O)O)O)OC)C |
| 存储条件 | -20°C干燥 |
| 运输条件 | 蓝冰 |
| SDF文件 | Download SDF |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | Bafilomycin A1是一种选择性的和可逆的空泡H+ ATPases（V-ATPases）抑制剂，IC50值介于4-400 nM之间。 |
| 靶点 | V-ATPases |
| 生物活性数据 | 4-400 nM |
生物相关数据
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产品引用文献
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- 9. Chunhua Shu, Rui Wang, et al. "The "Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Drug Delivery System Exerts Its Anti-tumor Effect by Inducing Lysosomal Membrane Permeability in the Process of Cell Death." Biol Pharm Bull. 2021 Oct 1;44(10):1473-1483. PMID: 34305072
- 10. Zining Wang, Feifei Xu, et al. "Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity." J Immunother Cancer. 2021 May;9(5):e002155. PMID: 34016722
- 11. Huiting Jia, Xindong Wang, et al. "Zinc(II)-Dipicolylamine Analogs Mediated PEI1.8k/pDNA Vector: Effect of Ligand Structure on the Gene Transport Process." Macromol Biosci. 2021 Jun;21(6):e2100048. PMID: 33861507
- 12. Zhuo Sun, Yidan Cao, et al. "Antiangiogenic effect of arsenic trioxide in HUVECs by FoxO3a‐regulated autophagy." J Biochem Mol Toxicol. 2021 Feb 16;e22728. PMID: 33592126
- 13. Wei Lei, Zhenyun Huo, et al. "Jervine inhibits non-small cell lung cancer (NSCLC) progression by suppressing Hedgehog and AKT signaling via triggering autophagy-regulated apoptosis." Biochem Biophys Res Commun. 2020 Dec 10;533(3):397-403. PMID: 32972750
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