规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1mL(10 mM in DMSO) | ¥1863.00 | 现货 | |
1mg | ¥454.00 | 现货 | |
5mg | ¥1818.00 | 现货 | |
10mg | ¥3181.00 | 现货 |
特色产品
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
产品描述
Staurosporine(星孢菌素)是一种由链霉菌Streptomyces staurospores产生的生物碱,最初是作为抗真菌剂使用的。Staurosporine是一种广谱性的蛋白激酶抑制剂,可以抑制蛋白激酶C(PKC)、cAMP依赖性蛋白激酶(PKA)、磷酸激酶、核糖体蛋白S6激酶、表皮生长因子受体(EGF-R)激酶和Ca2+/钙调蛋白依赖性蛋白激酶II(钙/钙调PKII)。Staurosporine对PKC的抑制作用是最强的(IC50 = 2.7 nM)。Staurosporine对一些哺乳动物的肿瘤细胞系表现出很强的细胞毒性,诱导细胞凋亡,抑制裂殖酵母在细胞分裂后的伸长。
参考文献:
Takashi Toda, Mizuki Shimanuki, and Mitsuhiro Yanagida. Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases. Genes Dev. 1991 5: 60-73
Michelle M. Hill, Mirjana Andelkovic, Derek P. Brazil, Stefano Ferrari, Doriano Fabbro, and Brian A. Hemmings. Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a Staurosporine-insensitive kinase. J. Biol. Chem 2001, 276: 25643-25646.
Flavio Meggio, Arianna Donella Deana, Maria Ruzzene, Anna M. Brunati, Luca Cesaro, Barbara Guerra, Thomas Meyer, Helmut Mett, Doriano Fabbro, Pascal Furet, Grazyna Dobrowolska, and Lorenzo A. Pinna. Different susceptibility of protein kinases to staurosporine inhibition kinetic studies and molecular bases for the resistance of protein kinase CK2. Eur. J. Biochem. 234, 317-322 (1995)
产品性质
物理外观 | A solid |
CAS号 | 62996-74-1 |
分子式 | C28H26N4O3 |
分子量 | 466.53 |
化学名称 | (5S,6R,7R,9R)-6-methoxy-5-methyl-7-(methylamino)-6,7,8,9,15,16-hexahydro-17-oxa-4b,9a,15-triaza-5,9-methanodibenzo[b,h]cyclonona[jkl]cyclopenta[e]-as-indacen-14(5H)-one |
溶解度 | insoluble in H2O; insoluble in EtOH; ≥11.66 mg/mL in DMSO |
SMILES | O=C(NC1)C2=C1C3=C(C4=C2C5=C(C=CC=C5)N4[C@H]6C[C@@H](NC)[C@@H](OC)[C@]7(C)O6)N7C8=CC=CC=C83 |
存储条件 | -20°C |
运输条件 | 蓝冰 |
SDF文件 | Download SDF |
产品应用 (实验数据来自文献,APExBIO并未验证,仅供参考)
IC50和靶点
生物活性描述 | Staurosporine是一种有效的PKC抑制剂,在非细胞试验中作用于PKCα、PKCγ 和PKCη,IC50分别为2 nM、5 nM 和 4 nM。 | |||||
靶点 | PKCα | PKCγ | PKCη | PKCδ | PKCε | PKCζ |
生物活性数据 | 2 nM | 5 nM | 4 nM | 20 nM | 73 nM | 1086 nM |
生物相关数据
质量控制
操作说明
产品引用文献
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