Catalog No. A1907
5-Azacytidine(别名:氮胞苷(5-氮胞苷))
DNA甲基转移酶抑制剂
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规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1mL(10 mM in DMSO) | ¥500.00 | 现货 | |
250mg(100 mM) | ¥500.00 | 现货 | |
1g | ¥1590.00 | 现货 |
CAS号:320-67-2纯度:98.00%
仅用于科研,不用于诊疗。未经明确授权不得转售。
A
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特色产品
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Cy5 TSA Fluorescence System Kit
Cy5荧光标记的酪胺信号放大系统
- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
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EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)
用于观察mRNA运送,定位,翻译
- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
产品描述
5-Azacytidine(5-AzaC),属于胞嘧啶类似物的化合物,是DNA甲基转移酶(DNMT)的抑制剂,对多发性骨髓瘤(MM)细胞,包括MM.1S、MM.1R、RPMI-8266、RPMI-LR5、RPMI-Dox40和患者来源的MM具有有效的细胞毒性,IC50值分别为1.5 μmol/L、0.7 μmol/L、1.1 μmol/L、2.5 μmol/L、3.2 μmol/L和1.5 μmol/L[1]。
5-Azacytidine可插入到细胞的DNA和/或RNA上,随后螯合DNMT,在5-Azacytidine的C6和DNMTs的半胱氨酸硫醇之间形成共价键,导致细胞中DNMT活性的缺失和DNA去甲基化[1]。
参考文献:
[1] Kiziltepe T, Hideshima T, Catley L, Raje N, Yasui H, Shiraishi N, Okawa Y, Ikeda H, Vallet S, Pozzi S, Ishitsuka K, Ocio EM, Chauhan D, Anderson KC. 5-Azacytidine, a DNA methyltransferase inhibitor, induces ATR-mediated DNA double-strand break responses, apoptosis, and synergistic cytotoxicity with doxorubicin and bortezomib against multiple myeloma cells. Mol Cancer Ther. 2007 Jun;6(6):1718-27.
产品性质
物理外观 | A solid |
CAS号 | 320-67-2 |
分子式 | C8H12N4O5 |
分子量 | 244.2 |
化学名称 | 4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,3,5-triazin-2-one |
溶解度 | insoluble in EtOH; ≥24.45 mg/mL in DMSO; ≥13.55 mg/mL in H2O with ultrasonic |
SMILES | C1=NC(=NC(=O)N1C2C(C(C(O2)CO)O)O)N |
存储条件 | -20°C |
运输条件 | 蓝冰 |
SDF文件 | Download SDF |
产品应用 (实验数据来自文献,APExBIO并未验证,仅供参考)
IC50和靶点
生物活性描述 | 5-Azacytidine是DNA甲基转移酶的抑制剂。 |
生物相关数据
质量控制
操作说明
产品引用文献
- 1. Dandan Li, Lingyun Xia, et al. "Serine protease PRSS56, a novel cancer-testis antigen activated by DNA hypomethylation, promotes colorectal and gastric cancer progression via PI3K/AKT axis." Cell Biosci. 2023 Jul 3;13(1):124. PMID: 37400936
- 2. Murakami S, Nemazanyy I, et al. "A Yap-Myc-Sox2-p53 Regulatory Network Dictates Metabolic Homeostasis and Differentiation in Kras-Driven Pancreatic Ductal Adenocarcinomas." Dev Cell. 2019 Aug 12. pii: S1534-5807(19)30628-8. PMID:31447265
- 3. Zhang W, Chen F, et al. "Zscan4c activates endogenous retrovirus MERVL and cleavage embryo genes." Nucleic Acids Res. 2019 Jul 15. pii: gkz594. PMID:31304534
- 4. Yang JR, Shi MX, et al. "LncRNA HAND2-AS1 inhibits proliferation and promotes apoptosis of chronic myeloid leukemia cells by sponging with micRNA-1275." Eur Rev Med Pharmacol Sci. 2019 Mar; 23 (5): 2103-2111. PMID:30915755
- 5. Li Z, Luo J. "Epigenetic regulation of HOTAIR in advanced chronic myeloid leukemia." Cancer Manag Res. 2018 Nov 5; 10: 5349-5362. PMID:30464631
- 6. Li Z, Yang L, et al. "Long noncoding RNA MEG3 inhibits proliferation of chronic myeloid leukemia cells by sponging microRNA21." Biomed Pharmacother. 2018 May 14; 104: 181-192. PMID:29772439
- 7. Li Z, Luo J. "Research on epigenetic mechanism of SFRP2 in advanced chronic myeloid leukemia." Biochem Biophys Res Commun. 2018 Jun 18; 501 (1): 64-72. PMID:29704505
- 8. Zhang X, Yang L, et al. "Research on the epigenetic regulation mechanism of the PTPN6 gene in advanced chronic myeloid leukaemia." Br J Haematol. 2017 May 8. PMID:28480959
- 9. Yagyu, Shigeki, et al. "Multiple mechanisms determine the sensitivity of human-induced pluripotent stem cells to the inducible caspase-9 safety switch." Molecular Therapy—Methods & Clinical Development 3 (2016).
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