WHI-P154
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
WHI-P154是酪氨酸激酶3(JAK3)的抑制剂，IC50值1.8 μM[1]。
体外激酶试验表明，WHI-P154不是一个特定的抑制剂。除了JAK3之外，它还能抑制许多其他激酶，包括EGFR、ABL、LCK、SRC和VEGFR。WHI-P154抑制EGFR的IC50值为4 nM。WHI-P154在细胞基础试验中也没有显示出特异性[1]。
在体外，WHI-P154对人胶质母细胞瘤细胞系U373表现出细胞毒性。抗肿瘤活性通过接合EGF而增强。在移植人胶质母细胞瘤的SCID鼠中，WHI-P154也显示了抗肿瘤活性[2]。
参考文献：
[1]. Changelian PS, Moshinsky D, Kuhn CF, Flanagan ME, Munchhof MJ, Harris TM, Whipple DA, Doty JL, Sun J, Kent CR, Magnuson KS, Perregaux DG, Sawyer PS, Kudlacz EM. The specificity of JAK3 kinase inhibitors. Blood. 2008 Feb 15;111(4):2155-7.
[2]. Narla RK, Liu XP, Myers DE, Uckun FM. 4-(3'-Bromo-4'hydroxylphenyl)-amino-6,7-dimethoxyquinazoline: a novel quinazoline derivative with potent cytotoxic activity against human glioblastoma cells. Clin Cancer Res. 1998 Jun;4(6):1405-14.
| Storage | Store at -20°C |
| M.Wt | 376.2 |
| Cas No. | 211555-04-3 |
| Formula | C16H14BrN3O3 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥125.6 mg/mL in DMSO |
| Chemical Name | 2-bromo-4-[(6,7-dimethoxyquinazolin-4-yl)amino]phenol |
| SDF | Download SDF |
| Canonical SMILES | COC1=C(C=C2C(=C1)C(=NC=N2)NC3=CC(=C(C=C3)O)Br)OC |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | WHI-P154是JAK3的抑制剂，IC50值1.8 μM。 | |||||
| 靶点 | JAK3 | |||||
| IC50 | 1.8 μM | |||||
质量控制和MSDS
- 批次:
化学结构
