tumor protein p53 binding protein fragment [Homo sapiens]/[Mus musculus]
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
P53是序列特异性的DNA结合寡聚蛋白,与含p53结合位点的启动子结合,激活转录。p53的活化区域位于氨基末端,目前没有研究报道特异性DNA结合结构域的位置1。
使用酵母双杂交筛选,鉴定到肿瘤蛋白p53结合蛋白(53BP1)可与P53的中心DNA结合结构域相互作用2。类似于乳腺癌易感基因1(BRCA1)3,4, 53BP1增强p53依赖性转录5。有趣的是,53BP1的羧基末端含有串联的BRCA1 COOH基端(BRCT)序列。
在电离辐射条件下,53BP1变得高度磷酸化,可迅速地重新定位于DNA链断裂的位点。在ATM缺陷细胞中,53BP1病灶形成减少,在ATM野生型细胞中,可以通过渥曼青霉素(wortmannin)抑制53BP1病灶形成。此外,使用渥曼青霉素处理的细胞以及ATM缺陷细胞中,不存在辐射诱导的53BP1过度磷酸化。53BP1参与DNA损伤信号传导途径,γ辐射后,可以被ATM调节。
Figure1. Tumor protein p53 binding protein 1
Figure2. Tumor protein p53 binding protein 2
参考文献:
1.J.Bargonetti, J. J. Manfredi et al. A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity wlien generated from wild-type but not from oncogenic mutant p53 protein, Genes Dev. 1993 7: 2565-2574
2.Iwabuchi, K., P.L. Bartel, B. Li, R. Marraccino, S. Fields (1994) Two cellular proteins that bind to wild-type but not mutant p53. Proc. Natl. Acad. Sci. USA91:6098–6102
3.Ouchi, T., A.N. Monteiro, A. August, S.A. Aaronson, H. Hanafusa (1998) BRCA1 regulates p53-dependent gene expression. Proc. Natl. Acad. Sci. USA95:2302–2306
4.Zhang, H., K. Somasundaram, Y. Peng, H. Tian, D. Bi, B.L. Weber, W.S. El-Deiry BRCA1 physically associates with p53 and stimulates its transcriptional activity Oncogene16199817131721
5.Iwabuchi, K., B. Li, H.F. Massa, B.J. Trask, T. Date, S. Fields(1998) Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2. J. Biol. Chem 273:26061–2606
6.Irene Rappold, Kuniyoshi Iwabuchi, Takayasu Date, and Junjie Chen Tumor Suppressor P53 Binding Protein 1 (53bp1) Is Involved in DNA Damage–Signaling Pathways. JCB vol. 153 no. 3 613-620
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1242.38 |
Formula | C57H87N13O18 |
Synonyms | H2N-Tyr-Leu-His-Val-Glu-Pro-Glu-Lys-Glu-Val-OH |
Solubility | ≥124.2 mg/mL in DMSO; ≥49.2 mg/mL in H2O; ≥50 mg/mL in EtOH |
SDF | Download SDF |
Canonical SMILES | OC(C=C1)=CC=C1CC(N)C(NC(CC(C)C)C(NC(CC2=CN=CN2)C(NC(C(C)C)C(NC(CCC(O)=O)C(N3CCCC3C(NC(CCC(O)=O)C(NC(CCCCN)C(NC(CCC(O)=O)C(NC(C(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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