Temozolomide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Temozolomide, an alkylating agent, is a DNA damage inducer.
- 1. Fanchu Zeng, Zhijin Fan, et al. "Tumor Microenvironment Activated Photoacoustic-Fluorescence Bimodal Nanoprobe for Precise Chemo-immunotherapy and Immune Response Tracing of Glioblastoma." ACS Nano. 2023 Oct 24;17(20):19753-19766. PMID: 37812513
- 2. Schwartz, Hannah, et al. "In vitro Methods to Better Evaluate Drug Responses in Cancer." UMass Chan Medical School. September 8, 2022.
Physical Appearance | A solid |
Storage | Store at -20°C, sealed storage, away from moisture and light |
M.Wt | 194.15 |
Cas No. | 85622-93-1 |
Formula | C6H6N6O2 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥29.61 mg/mL in DMSO |
Chemical Name | 3-methyl-4-oxoimidazo[5,1-d][1,2,3,5]tetrazine-8-carboxamide |
SDF | Download SDF |
Canonical SMILES | CN1C(=O)N2C=NC(=C2N=N1)C(=O)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
SK-LMS-1平滑肌肉瘤(MGMT- / p53 +),尤文肉瘤A-673和GIST-T1(两者具有MGMT + / p53-表型)和成胶质细胞瘤T98G(MGMT + / p53 +) |
溶解方法 |
在DMSO中的溶解度 >6.6 mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
62.5, 125, 250和500 μM; 72 h |
应用 |
在SK-LMS-1细胞中,Temozolomide抑制SK-LMS-1细胞的增殖活性。A-673细胞对temozolomide最敏感,其作用是时间和剂量依赖性的。尤文肉瘤细胞与O6-benzylguanine的预孵育增强了烷化剂的细胞毒性作用,降低了肿瘤细胞的活力。GIST-T1细胞对temozolomide不敏感。 |
动物实验[2]: | |
动物模型 |
PARP1野生型(WT)和PARP1敲除(KO)小鼠 |
剂量 |
68 mg/kg; 每天一次,持续5天; 口服 |
应用 |
在PARP1 WT小鼠中,与对照组相比,temozolomide显著降低肝脏中NAD +的浓度(22%,p = 0.02)。在PARP1 KO小鼠的肝脏中,与对照组相比,仅在temozolomide组中的NAD +具有统计学显著的降低(22%,p = 0.03)。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1] Khusnutdinov RR1, Boichuk SV2. Mechanisms of Sensitivity of Soft Tissue Sarcoma Cells to Temozolomide. Bull Exp Biol Med. 2017 Jul 18. [2]. Almeida GS1, Bawn CM1, Galler M1, et al. PARP inhibitor rucaparib induces changes in NAD levels in cells and liver tissues as assessed by MRS. NMR Biomed. 2017 May 22. |
质量控制和MSDS
- 批次: