T7 Endonuclease I
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T7 Endonuclease Ⅰ（T7EI）来源于大肠杆菌表达的重组T7 Endonuclease I基因，是一种特殊的DNA内切酶，能识别并切割不完全配对的DNA、十字型结构DNA、Holliday结构或交叉DNA和异源双链DNA，以及低速切割带缺刻位点的双链DNA。注意T7EI识别切割的插入、缺失或突变片段长度须≥2 bp，其切割位点为错配碱基5’端的第一、第二或第三个磷酸二酯键。本产品常用于CRISPR/Cas9、TALEN或以及其它基因编辑工具形成的突变体检测，基因点突变和SNP的检测，以及随机切割线性DNA进行shotgun克隆等。
| Components | 250 U | 1250 U |
| T7 Endonuclease I (10 U/μL) | 0.025 mL | 0.125 mL |
| 10X T7 Endonuclease I Reaction Buffer | 0.25 mL | 1.25 mL |
Store the components at -20 °C. | ||