T5 Exonuclease (RNase-free)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T5 Exonuclease是一种按照5'→3'方向降解双链或单链DNA的核酸外切酶。既能从双链或单链DNA 5'末端起始降解，也可以从线性或环状双链DNA的缺口(gap)或缺刻(nick)处起始降解，但不能降解超螺旋双链DNA。基于以上特性，T5 Exonuclease常被用于Gibson组装(Gibson Assembly)；降解线性单链、双链DNA或缺刻质粒DNA；从连接的环状双链DNA中去除不完全连接产物；降解线性和缺刻质粒DNA，以获得高纯度的超螺旋质粒DNA；去除碱裂法提取质粒过程中产生的变性质粒DNA；提高小量抽提质粒cDNA文库的转染效率。
| Components | 1000 U | 5000 U | 10000 U |
| T5 Exonuclease (RNase-free) | 0.1 ml | 0.5 ml | 1 ml |
| 10X T5 Exonuclease Reaction Buffer | 1 ml | 1 ml x5 | 1ml x10 |
Store at -20 °C. | |||