T4 DNA Ligase (RNase-free)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T4 DNA Ligase是一种分子量为55.3kDa的单链多肽,可催化双链DNA或RNA上相邻的5’磷酸和3’羟基末端形成磷酸二酯键,但对单链核酸无催化活性,其催化反应需ATP作为辅助因子。
T4 DNA连接酶适应性广,不仅可以将DNA片段与黏性末端或平末端结合,还可以修补双链DNA、双链RNA或DNA/RNA杂合体上的单链缺口,常用于DNA片段和载体、linker或adaptor的连接,缺口修复,以及线性 DNA 自环化等。
Components | 40 KU | 100 KU | 200 KU |
T4 DNA Ligase (400 U/μL) | 0.1 mL | 0.25 mL | 0.5 mL |
10X T4 DNA Ligase Reaction Buffer | 1 mL | 2.5 mL | 5 mL |
Store the components at -20 °C. |