survivin (baculoviral IAP repeat-containing protein 5) (21-28)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
生存素(杆状病毒含IAP重复凋亡抑制蛋白5)是来源于杆状病毒的细胞凋亡抑制剂,含有IAP repeat蛋白5,属于IAP基因家族,参与抑制细胞凋亡和调节有丝分裂。与健康组织相比,生存素是肿瘤组织中最均匀上调的基因之一。在多种类型癌细胞中,生存素的在原发肿瘤中的高表达与患者不良的预后反应相关,例如神经母细胞瘤3、大肠癌4、非小细胞肺癌5、B细胞白血病6、T细胞白血病7、肝细胞癌、食道癌、直肠癌、神经胶质瘤、膀胱癌、软组织肉瘤以及星形细胞瘤。
通过定量RT-PCR可以测定原发肿瘤中生存素mRNA的表达,测量发现在人类乳腺癌中,生存素具有强大的和独立的预后价值。生存素mRNA的浓度可用于将乳腺癌患者的不同风险进行分类8。过表达生存素的细胞,对凋亡信号的激活没有应答反应,针对细胞毒性治疗具有更强的抗性9。
Figure 1. Formula of Survivin (baculoviral IAP repeat-containing protein 5) (21-28)
Figure 2. Structure of Survivin (baculoviral IAP repeat-containing protein 5)
参考文献:
1.Altieri DC. Validating survivin as a cancer therapeutic target. Nat Rev Cancer 2003; 3:46 –54.
2.Velculescu VE, Madden SL, Zhang L, Lash AE, Yu J, Rago C, et al. Analysis of human transcriptomes. Nat Genet 1999; 23:387–8.
3.Adida C, Berrebi D, Peuchmaur M, Reyes-Mugica M, Altieri DC. Anti-apoptosis gene, survivin, and prognosis of neuroblastoma. Lancet 1998; 351:882–3.
4.Kawasaki H, Altieri DC, Lu CD, Toyoda M, Tenjo T, Tanigawa N. Inhibition of apoptosis by survivin predicts shorter survival rates in colorectal cancer. Cancer Res 1998; 58:5071–4.
5.Monzo M, Rosell R, Felip E, Astudillo J, Sanchez JJ, Maestre J, et al. A novel anti-apoptosis gene: Re-expression of survivin messenger RNA as a prognosis marker in non-small-cell lung cancers. J Clin Oncol 1999; 17:2100 –4.
6.Adida C, Haioun C, Gaulard P, Lepage E, Morel P, Briere J, et al. Prognostic significance of survivin expression in diffuse large B-cell lymphomas. Blood 2000; 96:1921–5.
7.Nakayama K, Kamihira S. Survivin an important determinant for prognosis in adult T-cell leukemia: a novel biomarker in practical hemato-oncology. Leuk Lymphoma 2002; 43:2249 –55.
8.Paul N. Span, Fred C.G.J. Sweep et al. Survivin Is an Independent Prognostic Marker for Risk Stratification of Breast Cancer Patients. Clinical Chemistry 50:11; 1986 –1993 (2004)
9.Zaffaroni N, Daidone MG. Survivin expression and resistance to anticancer treatments: perspectives for new therapeutic interventions. Drug Resist Updat 2002; 5:65–72.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1052.22 |
Formula | C54H73N11O11 |
Synonyms | H2N-Thr-Phe-Lys-Asn-Trp-Pro-Phe-Leu-OH |
Solubility | ≥105.2 mg/mL in DMSO; ≥47.6 mg/mL in EtOH; ≥58.3 mg/mL in H2O |
SDF | Download SDF |
Canonical SMILES | NC(C(C)O)C(NC(CC1=CC=CC=C1)C(NC(CCCCN)C(NC(CC(N)=O)C(NC(CC2=CNC3=C2C=CC=C3)C(N4C(C(NC(CC5=CC=CC=C5)C(NC(CC(C)C)C(O)=O)=O)=O)CCC4)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
![survivin (baculoviral IAP repeat-containing protein 5) (21-28)](http://www.apexbt.com//media/diy/images/struct/A1092.png)