SB590885
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB590885是有效的B-Raf激酶选择性抑制剂,其Ki值为0.16 nM[1].
SB590885是有效的致癌性B-Raf蛋白激酶抑制剂,其Ki值为0.16 nM.相对于C-Raf,SB590885更有效地抑制B-Raf.SB590885对C-Raf的Ki值为1.72 nM.SB590885具有高度选择性,对48种其它人类激酶无效,如Abl\AMPK\CK1\CK2以及ERK2.据发现,SB590885与B-Raf的ATP结合口袋结合,稳定B-Raf的活性构象.在表达B-Raf V600E致癌突变的肿瘤细胞中,SB590885减少ERK磷酸化并显示出抗增殖活性.除正常黑色素细胞和表达野生型B-Raf的原代黑色素瘤细胞外,正常细胞和不表达突变B-Raf的肿瘤细胞均对SB590885不敏感.此外,在表达突变B-Raf的恶性细胞中,SB590885还降低了其转化与致瘤性[1].
参考文献:
[1] King A J, Patrick D R, Batorsky R S, et al. Demonstration of a genetic therapeutic index for tumors expressing oncogenic BRAF by the kinase inhibitor SB-590885. Cancer research, 2006, 66(23): 11100-11105.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 453.55 |
Cas No. | 405554-55-4 |
Formula | C27H27N5O2 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥4.54 mg/mL in DMSO with gentle warming |
Chemical Name | N,N-dimethyl-2-[4-[(4Z)-4-(1-nitroso-2,3-dihydroinden-5-ylidene)-5-(1H-pyridin-4-ylidene)-1H-imidazol-2-yl]phenoxy]ethanamine |
SDF | Download SDF |
Canonical SMILES | CN(C)CCOC1=CC=C(C=C1)C2=NC(=C3C=CC4=C(CCC4=C3)N=O)C(=C5C=CNC=C5)N2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | SB590885是有效的B-Raf抑制剂,其Ki值为0.16 nM. | |||||
靶点 | B-Raf | |||||
IC50 | 0.16 nM |
质量控制和MSDS
- 批次:
化学结构
![SB590885](http://www.apexbt.com//media/diy/images/struct/B1406.png)