SB408124
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB408124是一种新型有效的非肽类食欲素-1(OX(1))受体拮抗剂,在细胞膜和全细胞中的Ki值分别为27 nM和57 nM.SB408124对OX1受体的选择性比人OX2受体高50倍[1].
在从大鼠大脑皮层中获得的原代星形细胞培养物中,使用食欲素A之前用SB408124预处理可显著降低食欲素A对毛喉素和基底诱导的cAMP生成的刺激活性[2].
在大鼠中,注射组胺(HA)或高盐水后,SB408124(30 μg/10 μl)与食欲素A共同给药可防止食欲素A诱导的对加压素(VP)浓度提高的减少[3].
参考文献:
[1] Langmead CJ1, Jerman JC, Brough SJ, Scott C, Porter RA, Herdon HJ. Characterisation of the binding of [3H]-SB-674042, a novel nonpeptide antagonist, to the human orexin-1 receptor. Br J Pharmacol. 2004 Jan;141(2):340-6.
[2] Woldan-Tambor A1, Biegańska K, Wiktorowska-Owczarek A, Zawilska JB. Activation of orexin/hypocretin type 1 receptors stimulates cAMP synthesis in primary cultures of rat astrocytes. Pharmacol Rep. 2011;63(3):717-23.
[3] Kis GK1, Molnár AH, Daruka L, Gardi J, Rákosi K, László F, László FA, Varga C. The osmotically and histamine-induced enhancement of the plasma vasopressin level is diminished by intracerebroventricularly administered orexin in rats. Pflugers Arch. 2012 Apr;463(4):531-6.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 356.38 |
Cas No. | 288150-92-5 |
Formula | C19H18F2N4O |
Solubility | insoluble in H2O; ≥1.76 mg/mL in EtOH with gentle warming and ultrasonic; ≥35.4 mg/mL in DMSO with gentle warming |
Chemical Name | 1-(6,8-difluoro-2-methylquinolin-4-yl)-3-[4-(dimethylamino)phenyl]urea |
SDF | Download SDF |
Canonical SMILES | CC1=NC2=C(C=C(C=C2C(=C1)NC(=O)NC3=CC=C(C=C3)N(C)C)F)F |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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