SB-3CT
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB-3CT是有效的明胶酶选择性抑制剂,对MMP-2和MMP-9的Ki值分别为13.9和600 nM.
明胶酶A(MMP-2)和B(MMP-9)属于基质金属蛋白酶家族,通过水解细胞外基质参与肿瘤转移和血管生成[1].
SB-3CT是一种有效的明胶酶选择性抑制剂.SB-3CT是一种新的基于机制的抑制剂,可直接结合MMP-2的催化锌离子[1].SB-3CT可抑制纯化的小鼠MMP-9,Ki值为120 nM[2].
在携带T细胞淋巴瘤L-CI.5s细胞的小鼠中,SB-3CT在50 mg/kg/d下可以剂量依赖的方式减少肝转移的数量,并可减少大于70%的肿瘤集落数量.SB-3CT(50 mg/kg/d)也可显著减小集落大小和肝脏PCNA阳性肿瘤细胞数量.这些结果表明SB-3CT可有效抑制肿瘤细胞溢出,并具有抗增殖效应.SB-3CT可通过抑制MMP-2和MMP-9,完全抑制MMP介导的溶明胶活性.并且,SB-3CT可增加存活率[2].在短暂局灶性脑缺血小鼠模型中,SB-3CT可抑制MMP-9介导的层粘连蛋白裂解,而后拯救神经元免于凋亡[2].
参考文献:
[1]. Kleifeld O, Kotra LP, Gervasi DC, et al. X-ray absorption studies of human matrix metalloproteinase-2 (MMP-2) bound to a highly selective mechanism-based inhibitor. comparison with the latent and active forms of the enzyme. J Biol Chem, 2001, 276(20): 17125-17131.
[2]. Krüger A, Arlt MJ, Gerg M, et al. Antimetastatic activity of a novel mechanism-based gelatinase inhibitor. Cancer Res, 2005, 65(9): 3523-3526.
[3]. Gu Z, Cui J, Brown S, et al. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia. J Neurosci, 2005, 25(27): 6401-6408.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 306.40 |
Cas No. | 292605-14-2 |
Formula | C15H14O3S2 |
Solubility | ≥30.6 mg/mL in DMSO; insoluble in H2O; ≥2.43 mg/mL in EtOH |
Chemical Name | 2-(((4-phenoxyphenyl)sulfonyl)methyl)thiirane |
SDF | Download SDF |
Canonical SMILES | O=S(CC1CS1)(C2=CC=C(OC3=CC=CC=C3)C=C2)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
![SB-3CT](http://www.apexbt.com//media/diy/images/struct/B4792.png)