S Tag Peptide
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
S Tag是胰核糖核酸酶A(RNase A)衍生的的寡肽。如果RNase A用枯草杆菌蛋白酶(subtilisin)消化,单肽键被裂解,得到两个保持弱结合的产物,该蛋白被称为核糖核酸酶S,仍具有活性,但两个产物单独不具有酶活性。原RNase A的N端,也称为S-肽,由20个氨基酸组成,只有前15个氨基酸是核糖核酸酶活性所必须的。这15个氨基酸的长肽被称为S15或S-tag。
据报道,富含带电和极性残基的多肽可改善它所连接蛋白的溶解性。而且,多肽单独不能折叠成独特的结构。在DNA水平,S-tag可以附着在任何蛋白的N端或C端。基因表达后,这种标签蛋白可通过商业化的抗体来检测[1]。
参考文献:
1. R.T. Raines et al., The S-Tag Fusion System for Protein Purification. Methods Enzymol. 326, 362-367 (2000)
- 1. Takushi Miyoshi, Qianli Zhang, et al. "Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures." Cell Rep. 2021 Feb 2;34(5):108708. PMID: 33535030
- 2. Hawa Gyamfi. "Protein Engineering of Microbial Ferritins." University of Waterloo. 2019.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 1748.91 |
Formula | C73H117N23O25S |
Synonyms | H-Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser-OH |
Solubility | ≥174.9 mg/mL in DMSO; insoluble in EtOH; ≥50 mg/mL in H2O |
Chemical Name | S Tag |
SDF | Download SDF |
Canonical SMILES | CC(C(C(=O)NC(C)C(=O)NC(C)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CC1=CC=CC=C1)C(=O)NC(CCC(=O)O)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCC(=O)N)C(=O)NC(CC2=CN=CN2)C(=O)NC(CCSC)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(CCCCN)N)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | S Tag Peptide是胰RNase A衍生的寡肽,用于改善蛋白质的溶解性。 | |||||
靶点 | anti-S-Tag antibody | |||||
IC50 |
质量控制和MSDS
- 批次:
化学结构
![S Tag Peptide](http://www.apexbt.com//media/diy/images/struct/A6007.png)