Recombinant Rat Interleukin-1 Receptor Antagonist Protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IL-1RA 最初被称为 IL-1 抑制剂，由 IL1RN 基因编码，是白细胞介素 1 细胞因子家族的成员。IL-1RA由多种类型的细胞分泌，包括免疫细胞、上皮细胞和脂肪细胞。IL-RA 具有通过与受体 IL1R1 结合并阻止其与共受体 IL1RAP 结合进行信号传导来抑制白细胞介素-1 活性的功能。IL-1RA还用于治疗类风湿性关节炎，这是一种自身免疫性疾病，IL-1在其中起关键作用。大鼠 IL-1RA 是一条含有 152 个氨基酸的非糖基化多肽链，已被证明在体外和体内阻断 IL-1 诱导的炎症反应。大鼠IL-1RA与小鼠和人IL-1RA具有89%和73%的a.a.序列同源性。
Reference
1. Dinarello CA. 1994. FASEB J. 8:1314-25
2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6
3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7
4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94
5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.
| Gene ID | 60582 |
| Accession # | P25086 |
| Alternate Names | IL-1RN, IRAP, IL1 Inhibitor |
| Source | Escherichia coli. |
| M.Wt | Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| AA Sequence | HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg in the presence of 50 pg/ml rRtIL-1α. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.