Recombinant Murine Endocrine Gland-derived Vascular Endothelial Growth Factor
Catalog No.
P1354
重组鼠内分泌腺源性血管内皮生长因子
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Source | Escherichia coli. |
M.Wt | Approximately 9.6 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
AA Sequence | AVITGACERD IQCGAGTCCA ISLWLRGLRL CTPLGREGEE CHPGSHKIPF LRKRQHHTCP CSPSLLCSRF PDGRYRCFRD LKNANF |
Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 3 % Trehalose. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. |
Shipping Condition | Gel pack. |
Handling | Centrifuge the vial prior to opening. |
Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
-
纯度 > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/μg of rMuEG-VEGF as determined by LAL method.