Recombinant Mouse IL25/IL17E, Tag Free
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IL-25,也被称为IL-17E,促进偏向Th2的免疫反应。这与其他IL-17家族成员促进Th1和Th17偏向的炎症形成了鲜明对比。IL-25是过敏反应和肠道寄生虫保护的重要介质[1, 2]。成熟小鼠IL-25与人和大鼠IL-25的氨基酸序列同源性分别为80%和91%[3, 4]。在蠕虫感染和过敏反应期间,IL-25在肠道和呼吸道上皮细胞、特应性皮炎皮肤损害、局部Th2细胞、嗜酸性粒细胞和嗜碱性粒细胞中局部上调[4-9]。它与IL-17RB结合,但也需要IL-17RA发挥其活性[3, 8, 10]。IL-25作用于多种类型的细胞,Th2细胞因子(如IL-4、IL-5、IL-13)的产生增加,Th1和Th17细胞因子(如干扰素-γ、IL-12、IL-23、IL-17A、IL-17F)的产生减少[4-6, 8, 9]。呼吸道IL-25可以被基质金属蛋白酶-7激活,基质金属蛋白酶-7是一种在呼吸道上皮细胞中上调的蛋白酶,对过敏原暴露做出反应。裂解后的IL-25与IL-17 Rb结合增强,Th2细胞因子诱生增强。Th2细胞因子反过来触发Th2记忆细胞和抗炎M2巨噬细胞的扩张,增加嗜酸性粒细胞的动员和激活,以及树突状细胞的迁移[4, 6, 9]。这些作用促进保护性抗蠕虫免疫反应[4, 5]以及过敏性炎症和呼吸道高反应性[11]。IL-25诱导的Th1和Th17细胞因子抑制限制了自身免疫和结肠炎中Th17细胞的扩张和疾病病理[12]。
Reference
[1]. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
[2]. Iwakura, Y. et al. (2011) Immunity 34:149.
[3]. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
[4]. Fort, M.M. et al. (2001) Immunity 15:985.
[5]. Zhao, A. et al. (2010) J. Immunol. 185:6921.
[6]. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
[7]. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
[8]. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
[9]. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
[10]. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
[11]. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
[12]. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161
Accession # |
Q8VHH8 |
Alternate Names |
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25 |
Source |
Human embryonic kidney cell, HEK293-derived mouse IL25/IL17E protein |
Protein sequence |
Val17-Ala169 |
M.Wt |
17.5 kDa |
Appearance |
Solution protein |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. |
Concentration |
0. 2 mg/mL |
Formulation |
Dissolved in sterile PBS buffer. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
Biological Activity |
The EC50 for this effect is 0.02-1.0 ng/mL. Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. |
Shipping Condition |
Shipping with dry ice. |
Handling |
Centrifuge the vial prior to opening. |
Usage |
For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
-
纯度 > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.