Recombinant Human Epidermal Growth Factor GMP
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
表皮生长因子(Epidermal Growth Factor, EGF)最初是在从小鼠颌下腺制备的神经生长因子的粗制剂中发现的,当注射到新生小鼠体内时,表皮生长因子会引起早期眼睑张开、门牙出疹、毛发生长抑制和生长发育迟缓。从尿中分离到人EGF,根据其抑制胃液分泌的作用,命名为尿胃泌素。EGF是来源于膜锚定前体的生长因子家族的原型。该家族的所有成员的特点是在其细胞外结构域存在至少一个EGF结构单元(由形成三个二硫键的保守6半胱氨酸基序的存在来定义)。EGF最初作为130 kDa的前体跨膜蛋白合成,含有9个EGF单位。成熟的可溶性EGF序列对应于位于跨膜结构域近端的EGF单元。膜EGF前体能够与EGF受体结合,据报道具有生物活性。成熟的人EGF与成熟的小鼠和大鼠EGF具有70%的a.a.序列一致性。
Reference
1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6
2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62
3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58
4. Cohen S. 2008. J Biol Chem, 283: 33793-7.
Gene ID | 1950 |
Accession # | P01133 |
Alternate Names | Urogastrone |
Source | Escherichia coli. |
M.Wt | Approximately 6.2 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids. |
AA Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107U/mg. |
Shipping Condition | Gel pack. |
Handling | Centrifuge the vial prior to opening. |
Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.01 EU/μg of rHuEGF GMP as determined by LAL method.