PMSF
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PMSF(苯甲基磺酰氟)是丝氨酸蛋白酶的一个不可逆抑制剂,与迟发性神经病的发展有关。它在许多类型的组织中对胞内修复和再生起作用。PMSF是一个长期作用的神经病靶标酯酶(NTE)抑制剂。NTE是一个保护剂,与迟发性多发性神经病(OPIDP)的假定靶点的抑制作用有关。PMSF可以将NTE抑制作用增加90%以上。PMSF还可作为γ-谷酰基转肽酶的一个活性位点定向试剂。
参考文献:
[1]. Thomas Baker, Herbert E. Lowndes, Martin K. Johnson, Irene C. Sandborg. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology. 1980; 46(3-4): 305 – 311.
[2]. Marcello Lotti, Stefano Caroldi, Eugenio Capodicasa, Angelo Moretto. Promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. Toxicology and Applied Pharmacology. 1991; 108(2): 234 – 241.
[3]. Masayasu Inoue, Seikoh Horiuchi, Yoshimasa Morino. Inactivation of γ-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. Biochemical and Biophysical Research Communications. 1978; 82(4): 1183 – 1188.
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Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 174.2 |
Cas No. | 329-98-6 |
Formula | C7H7FO2S |
Solubility | insoluble in H2O; ≥17.4 mg/mL in DMSO; ≥28.3 mg/mL in EtOH |
Chemical Name | phenylmethanesulfonyl fluoride |
SDF | Download SDF |
Canonical SMILES | C1=CC=C(C=C1)CS(=O)(=O)F |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
Longitudinal smooth muscle of guinea pig ileum |
Reaction Conditions |
2 mM PMSF for 30 min incubation |
Applications |
PMSF inhibited carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15% ~ 19%. The inhibition effect of PMSF on phosphoinositide turnover was due to one or more steps following phosphoinositide breakdown. |
Animal experiment:[2] | |
Animal models |
Cats |
Dosage form |
30 mg/kg Administered intraperitoneally (i.p.) |
Applications |
Pretreatment of cats with PMSF (30 mg/kg i.p.) 24 h before the diisopropylfluorophosphate (DFP) injection protected the cats from the delayed neuropathy. No clinical neurotoxic signs were observed at 21 days after DFP exposure. The stimulus-bound repetitive capacity of soleus α-motor nerve terminals was not lost at this time and its incidence was much greater than that which occurred in cats not pretreated with PMSF. |
Note |
The technical data provided above is for reference only. |
References: 1. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984, 5(3): 191-203. 2. Baker T, Lowndes HE, Johnson MK, et al. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology, 1980, 46(3-4): 305-311. |
质量控制和MSDS
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