Oligo (dT) 25 Beads
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
真核细胞内的mRNA仅占细胞总RNA的1%-5%,如何高效的提纯mRNA对后续实验至关重要。Oligo (dT) 25 Beads作为一款专门设计用于提纯真核mRNA的产品,采用特殊设计的单分散超顺磁珠,在其表面共价偶联寡聚dT序列,通过寡聚dT序列和mRNA的polyA尾之间的碱基配对,可以直接从真核生物总RNA或者细胞、动植物组织中快速分离高纯度的完整mRNA。
提取的mRNA可将结合的dT序列作为第一链反应引物合成cDNA,或者将mRNA从磁珠洗脱用于各类下游分子实验,如RT-PCR、RPA(核糖核酸酶保护试验)、文库构建、Northern Blot分析、二代测序等。
- 1. Mengjun Zhang, Zhuang Gu, et al. "SRRM2 phase separation drives assembly of nuclear speckle subcompartments." Cell Rep. 2024 Mar 26;43(3):113827. PMID: 38381607
- 2. Jiangnan Huang, Linjie Rao, et al. "Effect of crossbreeding and sex on slaughter performance and meat quality in Xingguo gray goose based on multiomics data analysis." Poult Sci. 2023 Aug;102(8):102753. PMID: 37267641
- 3. Pengyu Geng, Jinhui Zhao, et al. "Z-Ligustilide Combined with Cisplatin Reduces PLPP1-Mediated Phospholipid Synthesis to Impair Cisplatin Resistance in Lung Cancer." Int. J. Mol. Sci. 2023, 24, 17046.
Components | 1 mL | 5*1 mL |
Oligo (dT) 25 Beads (10 mg/mL) | 1 mL(10 mg) | 5*1 mL(50 mg) |
Store at 4 °C for 12-18 months, no freezing! |