O-propargyl-puromycin (OPP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
O-propargyl-puromycin(OPP)是嘌呤霉素的炔烃衍生物,用于监测新生蛋白质合成。其通过掺入翻译中多肽链的C-末端而使翻译停止,是一种有效的蛋白质合成抑制剂。
OPP可与新生多肽链缀合而构成共价形式,且可通过铜(I)催化的叠氮化物-炔烃环加成作用被可视化或捕获[1]。因此,OPP通常被认为是细胞和动物中新生蛋白质的成像和鉴定的标记物。已有研究报道,在CHO细胞中,OPP标记可以量化总蛋白质(global protein)的合成。此外,在单细胞水平,OPP标记使总蛋白质的合成率与DNA含量密切相关[2]。在体内研究中发现:注射OPP的小鼠组织与未注射OPP的小鼠组织相比,OPP与新生蛋白质的结合具有特异性 [1]。OPP介导的鉴定(OPP-ID)则是一种当细胞受到刺激和压力时研究细胞如何调节新生蛋白表达的技术,可应用于大多数细胞类型,用途广泛[3]。
References:
[1] Liu J, Xu Y, Stoleru D, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(2):413.
[2] Nagelreiter F, Coats M, Klanert G, et al. OPP labelling enables total protein synthesis quantification in CHO production cell lines at the single‐cell level [J]. Biotechnology Journal, 2018, 13(4):1700492.
[3] Forester C M, Zhao Q, Phillips N J, et al. Revealing nascent proteomics in signaling pathways and cell differentiation.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(10):2353-2358.
Storage | Store at -20°C |
M.Wt | 495.53 |
Cas No. | 1416561-90-4 |
Formula | C24H29N7O5 |
Solubility | Soluble in DMSO |
Chemical Name | (2S)-2-amino-N-((2S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-(prop-2-yn-1-yloxy)phenyl)propanamide |
SDF | Download SDF |
Canonical SMILES | OC[C@H]1O[C@@H](N2C3=NC=NC(N(C)C)=C3N=C2)C(O)C1NC([C@H](CC4=CC=C(OCC#C)C=C4)N)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |