O-Methylviridicatin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 2.5 μM: inhibits virus production in OM-10.1 cells (HL-60 promyelocytes infected with HIV-1) treated with TNF-α.
5 μM: blocks activation of the HIV long terminal repeat by TNF-α in HeLa cells
O-Methylviridicatin, a natural derivative of the alkaloid mycotoxin viridicatin, is produced by several species in the genus, Penicillium. It is demonstrated that O-Methylviridicatin, as an inhibitor, blocks the tumor necrosis factor alpha (TNFα)-induced replication of human immunodeficiency virus (HIV). TNFα increases the binding of the cellular transcription factor NF-κB to the HIV long terminal repeat (LTR), which directly triggers transcription of the latent provirus.
In vitro: O-Methylviridicatin had an inhibitory effect on the TNFα-triggered replication of HIV LTR in HeLa cells. In addition, O-Methylviridicatin dose-dependently dampened virus production in the OM-10.1 cell line, which was measured by the output of p24 core antigen into the culture medium. It was demonstrated that the suppressive effect of O-Methylviridicatin was not reversed even if the concentration of TNFα was increased 10-fold [1].
In vivo: Up to now, in vitro study of O-Methylviridicatin is still in the development stage.
Reference:
[1]. Heguy, A., Cai, P., Meyn, P., Houck, D., Russo, S., & Michitsch, R. et al. Isolation and Characterization of the Fungal Metabolite 3-O-Methylviridicatin as an Inhibitor of Tumour Necrosis Factor-Induced Human Immunodeficiency Virus Replication. Antiviral Chemistry and Chemotherapy. 1998; 9(2): 149-155.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 251.3 |
Cas No. | 6152-57-4 |
Formula | C16H13NO2 |
Synonyms | AIDS-089094,3-methoxy-4-phenyl-Carbostyril,Viridicatin methyl ester |
Solubility | Soluble in DMSO |
Chemical Name | 3-methoxy-4-phenyl-2(1H)-quinolinone |
SDF | Download SDF |
Canonical SMILES | O=C1NC2=C(C=CC=C2)C(C3=CC=CC=C3)=C1OC |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |