Neutral Red Cell Proliferation Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
中性红细胞增殖检测试剂盒（Neutral Red Cell Proliferation Assay Kit）是一种可以快速检测细胞增殖或毒性的试剂盒。细胞对中性红的摄入是由细胞维持pH梯度的能力来决定的，这一能力依赖于ATP的产生。在生理pH条件下，中性红可以通过被动扩散的方式进入细胞膜并累积在溶酶体中。当细胞增殖即细胞数量增加时，中性红的摄入量也会相应增加。反之，当细胞受损时，其摄入中性红的能力会减弱。通过测量细胞摄入中性红的量，可以评估细胞的增殖或毒性情况。
此外中性红还可以作为pH指示剂，其颜色会随pH值的变化而变化。中性红在酸性环境下呈现红色，而当pH值从6.8升至8.0时，其颜色会逐渐从红色变为黄色。
图1：本试剂盒的标准曲线
| Components |
K2262-500T |
|
Neutral Red Solution |
10 mL |
|
Neutral Red Lysis Buffer |
50 mL |
|
Store the kit at -20°C, stable for 1 year. Neutral Red Solution should be stored away from light. |
|