NADP+ (sodium salt hydrate)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NADP+ (sodium salt hydrate) is the oxidized form of the electron donor, reduced nicotinamide adenine dinucleotide phosphate (NADPH) [1].
NADP+ and NADPH are two fundamental common mediators involved in many biological processes, such as energy metabolism, mitochondrial functions, calcium homeostasis, antioxidation/generation of oxidative stress, gene expression, immunological functions, aging, and cell death. NADP+ and NADPH are mainly used by the enzymes that catalyze substrate reduction [1].
In cell extracts of human heteroploid cell lines HeLa and T-9, addition of NADP+ at 10-3 M stabilized glucose-6-phosphate dehydrogenase [2]. In air-dried and intact cells of Gluconobacter suboxydans, increasing concentrations of NADP+, up to 10 μmol/ml, led to increased NADPH production. However, NADP+ at concentrations more than 50 μmol/ml did not increase or markedly decrease the production of NADPH, which was considered to be due to acidification of the reaction mixture on the addition of a high concentration of free NADP+ and also on the formation of a large amount of gluconic acid via gluconolactone [3].
References:
[1]. Ying W. NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences. Antioxidants & Redox Signaling, 2008, 10(2): 179-206.
[2]. Alekseev S B, Mamaev V B, Zgurskii A A, et al. Stability of glucose-6-phosphate dehydrogenase in human cells cultivated in vitro. Biokhimiia, 1979, 44(6): 1093-1100.
[3]. Izumi Y , Nath P K , Yamamoto H , et al. NADPH production from NADP+ with a glucose dehydrogenase system involving whole cells and immobilized cells of Gluconobacter suboxydans. Applied Microbiology and Biotechnology, 1989, 30(4): 337-342.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 783.4 |
Cas No. | 698999-85-8 |
Formula | C21H29N7NaO18P3 |
Solubility | insoluble in DMSO; insoluble in EtOH; ≥51.1 mg/mL in H2O |
SDF | Download SDF |
Canonical SMILES | O[C@H]1[C@H]([C@@H](O[C@@H]1COP(OP(OC[C@@H]2[C@H]([C@H]([C@@H](O2)[N+]3=CC(C(N)=O)=CC=C3)O)O)([O-])=O)([O-])=O)N4C=NC5=C4N=CN=C5N)OP(O)(O)=O.[Na+].O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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