N1-Methylpseudouridine
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
N1-Methylpseudouridine is a modified nucleoside used for enhancing mRNA translation. Incorporation of N1-Methylpseudouridine into mRNA turns off the immune/eIF2α phosphorylation-dependent inhibition of translation, and increases ribosome pausing and density on the mRNA, thus dramatically facilitating the translation process. N1-Methylpseudouridine outperforms several other modified nucleosides (e.g. 5-Methylcytidine) and corresponding combinations in terms of translation capacity.
References:
1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036.
2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 258.23 |
Cas No. | 13860-38-3 |
Formula | C10H14N2O6 |
Solubility | ≥50 mg/mL in H2O with ultrasonic; ≥20 mg/mL in EtOH; ≥20.65 mg/mL in DMSO |
Chemical Name | 5-((2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-1-methylpyrimidine-2,4(1H,3H)-dione |
SDF | Download SDF |
Canonical SMILES | O=C(N1)N(C)C=C([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C1=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[2] | |
Cell lines |
Various mammalian cells including A549, BJ, C2C12, HeLa and primary keratinocytes |
Reaction Conditions |
24 h incubation |
Applications |
In various mammalian cells, mRNAs in the N1-Methylpseudouridine + 5-Methylcytidine group showed reduced cytotoxicity compared to the Pseudouridine + 5-Methylcytidine group. The mRNAs simultaneously modified by N1-Methylpseudouridine and 5-Methylcytidine also exhibited reduced activation of the intracellular innate immune response. |
Animal experiment:[2] | |
Animal models |
7-week-old Balb/c mice |
Dosage form |
20 μg Once daily through intradermal or intramuscular routes by lipofection, for 21 days |
Applications |
N1-Methylpseudouridine alone and its combination with 5-Methylcytidine outperformed the current state-of-the-art Pseudouridine and its combination with 5-Methylcytidine, in terms of translation capacity. |
Note |
The technical data provided above is for reference only. |
References: 1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036. 2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344. |
质量控制和MSDS
- 批次:
化学结构
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