MTT
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50:N/A。
MTT(3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-溴化四唑)是一种用于检测体外细胞增殖的试剂。
在细胞生物学中,四唑盐已被广泛用于检测从微生物细胞到哺乳动物细胞的代谢活性。哺乳动物细胞的组分研究表明,NADH(还原型吡啶核苷酸辅因子)能使大多数MTT还原,全细胞研究也进一步证明上述结论。
体外:MTT还原不仅与线粒体相关,同时也与细胞质以及非线粒体膜(包括核内体膜、溶酶体膜和质膜)相关。MTT所带的净正电荷是通过质膜电位参与其细胞摄取过程的主要因子。然而,第2代四唑染料(XTT、WST-1以及MTS)形成水溶性formazans,需要用于还原反应的中间电子受体。由于第2代四唑染料携带一个净负电荷,因而其细胞渗透性极差[1]。
体内:目前,MTT仅用于细胞体外增殖测定,暂无体内研究。
参考文献:
[1] Berridge MV, Herst PM, Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.
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Storage | Store at -20°C |
M.Wt | 414.32 |
Cas No. | 298-93-1 |
Formula | C18H16N5SBr |
Solubility | ≥41.4 mg/mL in DMSO; ≥18.63 mg/mL in EtOH; ≥2.5 mg/mL in H2O with ultrasonic |
Chemical Name | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide |
SDF | Download SDF |
Canonical SMILES | CC1=C(C)N=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)S1.[Br-] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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