MTS Cell Proliferation Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
MTS细胞增殖检测试剂盒(MTS Cell Proliferation Assay Kit)是一种常见的细胞增殖与毒性检测试剂盒。MTS,一种新型四唑盐,可以被活细胞线粒体内的琥珀酸脱氢酶还原成溶于水的产物甲臜(Formazan)。甲臜在490 nm处有特异性吸收,所以可以通过测定490 nm处的吸光度反映细胞增殖情况。细胞增殖越快,吸光度值越高。
与常见的MTT试剂盒相比,本试剂盒无需去除原有培养基,也无需另外使用溶剂溶解甲臜,使用更方便,同时可以避免去除培养基时带走部分甲臜而引起的实验误差。本试剂盒使用非常灵活,检测后可继续孵育,以得到更好的显色结果。此外,本试剂盒还具有线性范围宽、灵敏度高、使用安全等优点。
图1:本试剂盒的参考标准曲线
Components | K2250-500T | K2250-2500T |
MTS Solution | 10 mL | 50 mL |
PMS Solution | 0.5 mL | 2.5 mL |
Store the kit at -20°C away from light and moisture, stable for 1 year. For frequent use, the kit can be stored at 4°C away from light and moisture, stable for 6 weeks. |