ML-291
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ML-291 is an apoptosis inducer.
Apoptosis, a process of programmed cell death, occurs in multi-cellular organisms. Biochemical events result in characteristic cell changes (morphology) and death. These changes include cell shrinkage, nuclear fragmentation, blebbing, chromatin condensation, chromosomal DNA fragmentation, as well as global mRNA decay.
In vitro: ML-291 was identified as a novel activator of the apoptotic arm of the unfolded protein response (UPR), but not the adaptive arm. Specifically, ML-291 could activate signaling via PERK/eIF2α/CHOP with EC50 value of 762 nM but not through IRE1/XBP1. Moreover, ML-291 induced apoptosis in mouse embryonic fibroblasts overexpressing CHOP, but not in wild-type or CHOP knockout cells. ML-291 showed minimal activity against a panel of 67 receptors, ion channels, as well as transporters, with the exception of the dopamine transporter with 68% inhibition. In addition, ML-291 showed greater cytotoxicity than average antitumor cell cytotoxicity against colon, melanoma, and renal cancer cell lines in an NCI-60 panel [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Flaherty, D. P.,Golden, J.E.,Liu, C., et al. Selective small molecule activator of the apoptotic arm of the UPR. Probe Reports from the NIH Molecular Libraries Program (2012).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 413.8 |
Cas No. | 1523437-16-2 |
Formula | C16H16ClN3O6S |
Solubility | ≤1mg/ml in DMSO;10mg/ml in dimethyl formamide |
Chemical Name | N-[4-[(4-chloro-1-piperidinyl)sulfonyl]phenyl]-5-nitro-2-furancarboxamide |
SDF | Download SDF |
Canonical SMILES | ClC1CCN(S(C2=CC=C(NC(C3=CC=C([N+]([O-])=O)O3)=O)C=C2)(=O)=O)CC1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |