Live-Dead Cell Staining Kit
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Yeyang Wang, et al. "Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering." Sci Rep. 2024 Feb 16;14(1):3942. PMID: 38365964
- 2. Junyu Chen, et al. "Magnetic scaffold constructing by micro-injection for bone tissue engineering under static magnetic field." Journal of Materials Research and Technology. Volume 29, March–April 2024, Pages 3554-3565.
- 3. Tanchen Ren, et al. "Enhancing aortic valve drug delivery with PAR2-targeting magnetic nano-cargoes for calcification alleviation." Nat Commun. 2024 Jan 16;15(1):557. PMID: 38228638
- 4. Chao Zhang, Hong Huang, et al. "DNA supramolecular hydrogel-enabled sustained delivery of metformin for relieving osteoarthritis." ACS Appl Mater Interfaces. 2023 Apr 5;15(13):16369-16379. PMID: 36945078
我们的产品Live-Dead Cell Staining Kit被用于同时荧光染色活细胞和死细胞。该试剂盒包含两种染料-钙黄绿素-AM (Calcein-AM) 和碘化丙锭 (PI),分别染色活细胞和死细胞。 Calcein-AM是Calcein的乙酰氧基甲酯,具有高度亲脂性,且可渗透细胞膜。尽管Calcein-AM本身不是荧光分子,但在经过活细胞中的酯酶消化后,它将转化为Calcein,发出强烈的绿色荧光 (最大激发波长:490 nm,最大发射波长:515nm)。因此,Calcein-AM仅染色活细胞。
相反,PI(一种核染色染料)不能穿过活细胞膜。它能够穿过死细胞膜的无序区域到达细胞核,并插入细胞核的DNA双螺旋中,发出红色荧光(最大激发波长:535 nm,最大发射波长:617 nm)。
由于calcein和PI-DNA均可在490 nm处激发,因此可以通过荧光显微镜或流式细胞仪同时监测活细胞和死细胞。在545 nm激发下,只有死细胞才能发出红色荧光。由于最佳的染色条件会因细胞系的不同而变化,因此我们建议应分别确定PI和Calcein-AM的合适浓度。
请注意,PI被怀疑具有高度致癌性;需要谨慎处理。
500 Tests | 1000 Tests | |
Calcein-AM Solution (2 mM) | 50 μL | 50 μL × 2 |
PI Solution (1.5 mM) | 150 μL | 150 μL × 2 |
储存于-20°C,避光。 Calcein-AM容易被水分水解。请保持干燥,使用后盖紧盖子。 |