Lipid Hydroperoxide (LPO) Assay Kit with 96-well plate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
脂质过氧化是评估氧化应激水平的重要指标之一，其是细胞内的脂质（饱和和不饱和脂质）在自由基作用下发生氧化分解形成各种产物的复杂过程。以往脂质过氧化主要是通过多不饱和脂肪酸(PUFA)的分解产物丙二醛(MDA)和4-羟基壬烯醛(4-HNE)来检测的。使用这两种方法进行检测都存在一定局限性，因为只有来自PUFA的脂质过氧化才会产生这些产物，这可能会导致检测结果不准确。鉴于这些检测的局限性，直接测量脂质过氧化物是更好的选择。
脂质过氧化检测试剂盒（Lipid Hydroperoxide (LPO) Assay Kit with 96-well plate）是一种优化过的，利用与亚铁离子的氧化还原反应直接检测脂质过氧化物的试剂盒。脂质过氧化物高度不稳定，容易与亚铁离子反应生成铁离子，而铁离子可以通过硫氰酸根离子作为显色剂进行检测。但是许多样本中可能含有铁离子，这会对检测造成干扰。本试剂盒进行优化，用氯仿抽提样本中的过氧化产物，从而可以实现在去除样本中铁离子干扰的情况下进行检测。与K2265相比，本试剂盒提供可供实验使用的96孔板，无需另外自行准备，使用更方便。
图1：本试剂盒的参考标准曲线
| Components |
K2266-100 T |
|
LPO Assay FTS Reagent 1 |
3 mL |
|
LPO Assay FTS Reagent 2 |
3 mL |
|
Lipid Hydroperoxide Standard |
3.5 mL |
|
LPO Assay Extract R |
1 vial |
|
LPO Assay Triphenylphosphine |
1 vial |
|
96-well Glass-Coated Plastic Well Plate |
1 plate |
|
Foil Plate Cover |
1 cover |
|
Store Lipid Hydroperoxide Standard at -80°C, the remaining reagents should be stored at 4°C, 96-well Plate and Cover can be stored at room temperature. This kit is stable for 1 year. |
|