HyperScript™ IV RT SuperMix for qPCR (with gDNA wiper)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScript™ IV Reverse Transcriptase是在M-MLV Reverse Transcriptase 基础上经过基因工程改造后获得的第四代逆转录酶,在逆转录反应中具有强大的扩增能力和可靠性,该酶在抑制剂抵抗性、延伸能力和反应速度方面得到了显著改善,同时保留了第三代逆转录酶的优点,包括热稳定性、高效全长cDNA合成和降低的RNase H活性。本产品可用于在抑制剂存在的情况下(RNA提取过程的残留)提供可靠,持续,快速的cDNA合成。
HyperScript™ IV RT SuperMix for qPCR适用于两步法RT-qPCR检测,5×HyperScript™ IV RT SuperMix中含有逆转录所需的所有成分,加入模板RNA和RNase Free ddH2O就可以迅速进行反应。产品中包含的4×gDNA wiper可在逆转录前快速去除体系中潜在的基因组DNA干扰。
本产品针对qPCR进行了特别优化,包括Oligo (dT)23VN primer /Random primer比例优化,使cDNA合成可从RNA转录本的各个区域起始并具有相同的逆转录效率,最大程度保证了qPCR结果的真实性和可重复性。逆转录产物适用于SYBR Green和探针法qPCR,您可以根据实验目的,选择相应的试剂。
Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
RNase Free ddH2O | 1 ml | 2×1 mL |
4×gDNA wiper mix | 200 μL | 400 μL |
5×HyperScript™ IV RT SuperMix | 200 μL | 400 μL |
5×HyperScript™ IV No RT control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. |