HyperScript™ III RT SuperMix for qPCR (with gDNA wiper)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScript™ III Reverse Transcriptase是在M-MLV Reverse Transcriptase基础上经过基因工程改造后获得的第三代全新逆转录酶。相较野生型和其他版本而言HyperScript™ III Reverse Transcriptase降低了RNase H活性，提高了热稳定性和保真度。进而具有更高的cDNA合成产量和长度，更高的对于高GC含量RNA的逆转录效率和整体更好的性能等特点。HyperScript™ III Reverse Transcriptase增强了与模板的亲和力，适合少量模板以及低拷贝基因的逆转录，可扩增长达12.3 kb的cDNA。
HyperScript™ III RT SuperMix for qPCR (with gDNA wiper)适用于两步法qRT-PCR检测，5×HyperScript™ Ⅲ SuperMix中含有逆转录所需的所有成分，加入模板RNA和RNase Free ddH2O就可以迅速进行反应。产品中包含的4×gDNA wiper可在逆转录前快速彻底去除体系中潜在的基因组干扰。该试剂盒非常适合于低浓度RNA模板的逆转录反应。5X HyperScript™ Ⅲ SuperMix在-20℃不会冻结，使用方便。
本产品针对qPCR进行特别优化，经过比例优化的Oligo (dT)23VN primer /Random primers mix，使cDNA合成可从RNA转录本的各个区域起始并具有相同的逆转录效率，最大程度保证了qPCR结果的真实性和可重复性。逆转录产物适用于SYBR Green和探针法qPCR，可以根据实验目的，选择相应的试剂配合使用，进行高性能的基因表达分析。
| Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
| RNase Free ddH2O | 1 mL | 2×1 mL |
| 4×gDNA wiper mix | 200 μL | 400 μL |
| 5×HyperScript™ Ⅲ RT SuperMix | 200 μL | 400 μL |
| 5×HyperScript™ Ⅲ No RT control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. | ||