HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, 5-moUTP, T7)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribe™共转录mRNA合成试剂盒(ARCA、5-moUTP、T7)专为体外快速生产含ARCA加帽的mRNA而设计。通过使用T7 RNA聚合酶共转录掺入抗反向帽类似物(ARCA,目录号B8175)使mRNA加帽,ARCA加帽确保mRNA的高翻译效率,添加5-moUTP(货号B8061)可降低宿主细胞免疫反应。另外,poly(A)尾能稳定真核生物mRNA,并提高翻译起始效率。本试剂盒推荐使用3 ’端自带加尾序列的DNA转录模板进行mRNA的poly(A)尾稳定添加(通常为100-120 A)。
使用该试剂盒合成的RNA在生物学实验中有许多应用,例如体外翻译,反义RNA和RNAi实验,RNA疫苗,RNA结构和功能研究,核酶生物化学,RNase蛋白实验和基于探针的杂交印迹。
该试剂盒包含足够的试剂,可进行25次反应,每次20 μL,并在K1065的基础上调整了组分及反应配比。对于标准20 μL反应体系可获得更高的RNA产量。
Components | 25 rxn |
T7 RNA Polymerase Mix | 50 μL |
10 × Reaction Buffer | 50 μL |
ATP (100 mM) | 30 μL |
5-moUTP (100 mM) | 25 μL |
CTP (100 mM) | 25 μL |
GTP (100 mM) | 25 μL |
ARCA (100 mM) | 20 μL |
Control Template (0.5 μg/μL) | 5 μL |
RNase-free H2O | 0.5 mL |
Store the components at -20°C. |