HotStart™ Universal 2X FAST SYBR Green qPCR Master Mix
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
定量PCR(qPCR,又称Real-time PCR)是一种非常通用的精确分析基因表达的技术。按方法不同可分为染料法和探针法两类,其中染料法更通用、方便、成本更低。基于染料的qPCR法通过实时监测结合双链DNA的染料发射的荧光,可以在PCR的每个周期间接测量DNA扩增。当在某一个时间点上,检测到的荧光信号显著超过背景,就可以确定Ct值(Cq值)。所获得的Ct值可用于评估目标基因的相对丰度,也可根据适当的标准曲线计算获得绝对数量。
我们的产品HotStartTM Universal 2X FAST SYBR Green qPCR Master Mix在定量目标DNA或cDNA方面具有较短的延伸时间、优越的特异性、强劲的扩增效率、理想的可重复性和稳定性。它是一个2X PreMix,其中的酶为经过突变改造而成的热启动快速Taq DNA聚合酶,可更耐受SYBR Green I的抑制,同时也更加耐受EDTA处理的血液和肝素处理的血液。理想的Taq聚合酶和合适的缓冲液保证了较好的特异性和较高的扩增速度。Mix中的SYBR Green I可嵌入双链DNA的双螺旋小沟区域,当它与每个周期扩增的双链DNA结合时,会发出绿色荧光,通过仪器监测荧光可以实时的间接定量扩增产物。
本产品专为快速实时PCR而设计开发的新型产品,提高了耐用性、特异性和延伸速度,并在信噪比(荧光)、循环域值 (Cq)、线性度和灵敏度方面均有改善。同时,产品中含有特殊的Specific ROX Reference Dye,适用于所有的qPCR仪器,无需在不同的仪器上调整ROX的浓度,只需在配置的反应体系时加入引物和模板既可进行扩增。
染料法qPCR有一定的局限性,即SYBR Green I可以插入任何双链DNA,如引物二聚体或其他非特异性的产物,导致非特异性产物发出荧光,因此为了确认产物的特异性,在扩增后,进行融解曲线分析是必要的。在融解曲线分析中,在引物退火温度附近出现一个尖峰是较为理想的实验结果。
Components | 5 mL 1000 rxn with 10 μL reaction 500 rxn with 20 μL reaction 200 rxn with 50 μL reaction |
25 mL 5000 rxn with 10 μL reaction 2500 rxn with 20 μL reaction 1000 rxn with 50 μL reaction |
50 mL 10000 rxn with 10 μL reaction 5000 rxn with 20 μL reaction 2000 rxn with 50 μL reaction |
HotStart™ Universal 2X FAST SYBR Green qPCR Master Mix | 1 mL X 5 | 1 mL X 25 | 5 mL X 10 |
Store the components away from light at -20°C for 12-24 months. |