Ginkgolic Acid C15:1
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ginkgolic acid is an alkylphenol derivative that causes allergic skin inflammation. IC50 values of ginkgolic acid against the SUMOylation of RanGAP1-C2 are 3.0 μM. Ginkgolic Acid, a Major Component of Ginkgo biloba Extract, inhibited SUMOylation in vitro and in vivo [1].
The cytotoxicity of ginkgolic acid (15:1) in primary rat hepatocytes was lower than in HepG2 cells. Ginkgolic acid (15:1) was demonstrated less cytotoxicity in four-day-cultured primary rat hepatocytes than in 20-h cultured ones. Co-incubation with selective CYP inhibitors, α-naphthoflavone and ketoconazole, could decrease the cytotoxicity of ginkgolic acid (15:1) in primary rat hepatocytes. In agreement, pretreatment with selective CYP inducers, β-naphthoflavone and rifampin, could increase the cytotoxicity of ginkgolic acid (15:1) in HepG2 cells [2]. Ginkgolic acid inhibited the growth of tumorogenic cell lines in a dose- and time-dependent manner. Tumor cells were treated with GA for 72 h, 70.53 ± 4.54% Hep-2 and 63.5 ± 7.2% Tca8113 cells were retarded at GO/G1 phase, and the percentage of apoptosis was 40.4 ± 1.58 and 38.4 ± 1.7%, respectively [3]. In 293T cells expressing Flag-tagged SUMO, ginkgolic Acid Inhibited SUMOylation. Ginkgolic acid impaired SUMOylation by blocking the formation of an E1-SUMO thioester complex, by directly binding to E1 [1].
References:
[1] Fukuda I, Ito A, Hirai G, et al. Ginkgolic acid inhibits protein SUMOylation by blocking formation of the E1-SUMO intermediate[J]. Chemistry & biology, 2009, 16(2): 133-140.
[2] Liu Z H, Zeng S. Cytotoxicity of ginkgolic acid in HepG2 cells and primary rat hepatocytes[J]. Toxicology letters, 2009, 187(3): 131-136.
[3] Zhou C, Li X, Du W, et al. Antitumor effects of ginkgolic acid in human cancer cell occur via cell cycle arrest and decrease the Bcl-2/Bax ratio to induce apoptosis[J]. Chemotherapy, 2010, 56(5): 393-402.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 346.5 |
Cas No. | 22910-60-7 |
Formula | C22H34O3 |
Synonyms | Anacardic Acid 15:1,Ginkgolic Acid I |
Solubility | insoluble in H2O; ≥44 mg/mL in EtOH; ≥46 mg/mL in DMSO |
Chemical Name | 2-hydroxy-6-(8Z)-8-pentadecenyl-benzoic acid |
SDF | Download SDF |
Canonical SMILES | OC1=CC=CC(CCCCCCC/C=C\CCCCCC)=C1C(O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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