Fluo-4 AM
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Fluo-4 AM是最常见的用于细胞内Ca2+浓度检测的荧光探针之一。Fluo-4 AM是Fluo-4的乙酰甲酯衍生物，可以穿透细胞膜，并被细胞内的酯酶剪切形成Fluo-4。Fluo-4可以滞留在细胞内并与细胞内Ca2+结合，然后发出明亮的荧光。Fluo-4 AM是在Fluo-3 AM的基础上改造而成，其优势在于加载速度更快且在同等条件下荧光更明亮。Fluo-4 AM将Fluo-3 AM结构中的氯离子替换成了氟原子，导致最大激发波长向短波长偏离12 nm左右，这个波长更接近氩激光器的波长（488 nm），导致氩激光器激发时Fluo-4比Fluo-3的荧光强1倍。同时Fluo-4结合Ca2+产生的荧光强度比紫外激发的Fura-2和Indo-1探针强至少100倍。本产品以溶于DMSO的2 mM储存液的形式提供。
| Ex/Em | 494/516（结合Ca2+后） | 储存条件 | -20°C干燥避光保存 |
| 运输条件 | 蓝冰 |
| 保质期 | -20°C干燥避光保存，避免反复冻融，6个月有效。 |
| Physical Appearance | Liquid |
| M.Wt | 1096.95 |
| Cas No. | 273221-67-3 |
| Formula | C51H50F2N2O23 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Components | B8807-20 µL |
| Fluo-4 AM | 20 µL (2 mM) |
This product should be stored at -20°C away from light and moisture. Avoid repeated freeze/thaw cycles, stable for 6 months. | |