EZ Cap™ Mouse p53 mRNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
p53是研究最多的转录因子之一，可以响应致癌信号激活其下游基因，如促凋亡蛋白BAX，上调凋亡调节因子PUMA等，p53还作为细胞周期检查点保护剂诱导细胞周期停滞，参与DNA复制和修复，保护基因组完整性。
EZ Cap™ Mouse p53 mRNA的浓度约为1 mg/mL。通过共转录加帽生成Cap 1结构。 Cap 1 结构更适合哺乳动物系统，并且比 Cap 0结构（ARCA和mCap）具有更高的转录效率。Poly(A) tail的添加增加了mRNA在体外和体内的稳定性和寿命。Poly(A) tail 在提高翻译启动效率方面也起着重要作用。
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mRNA Length |
1428 nucleotides |
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Concentration |
~1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融，不要涡旋震荡。首次使用时，将其轻柔离心并分成几份，可供单独使用。使用不含RNase的试剂和耗材，使用适当的无RNase技术。直至与转染试剂混合，才可加入含有血清的培养基中。 |
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Shipping Condition |
干冰运输 |
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