EZ Cap™ Mouse p53 mRNA (5-moUTP)
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
p53是研究最多的转录因子之一,可以响应致癌信号激活其下游基因,如促凋亡蛋白BAX,上调凋亡调节因子PUMA等,p53还作为细胞周期检查点保护剂诱导细胞周期停滞,参与DNA复制和修复,保护基因组完整性。
EZ Cap™ Mouse p53 mRNA (5-moUTP)的浓度约为1 mg/mL。通过共转录加帽生成Cap 1结构。Cap 1结构更适合哺乳动物系统,并且比Cap 0结构(ARCA和mCap)具有更高的转录效率。5-moUTP和poly (A) tail抑制RNA介导的先天免疫激活,并增加mRNA在体外和体内的稳定性和寿命。poly (A) tail 在提高翻译启动效率方面也起着重要作用。
mRNA Length |
1428 nucleotides |
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Concentration |
~1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
General tips |
请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融,不要涡旋震荡。首次使用时,将其轻柔离心并分成几份,可供单独使用。使用不含RNase的试剂和耗材,使用适当的无RNase技术。直至与转染试剂混合,才可加入含有血清的培养基中。 |
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Shipping Condition |
干冰运输 |