EZ Cap™ Cre mRNA (ΨUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cre重组酶是一种酪氨酸重组酶，可识别并催化DNA两个loxP位点之间的同源重组。该mRNA表达Cre重组酶，使用共转录方式加帽，具有Cap 1结构，更适合哺乳动物系统，并具有更高的表达效率，同时mRNA被聚腺苷酸化，并添加假尿苷 (ΨUTP)修饰，可以减少宿主细胞的免疫反应，并提高稳定性和翻译效率。
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mRNA Length |
1282 nucleotides |
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Concentration |
1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融，不要涡旋震荡。首次使用时，将其轻柔离心并分成几份，可供单独使用。使用不含RNase的试剂和耗材，使用适当的无RNase技术。直至与转染试剂混合，才可加入含有血清的培养基中。 |
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Shipping Condition |
干冰运输 |
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