(-)-epicatechin
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
(-)-epicatechin是一种类黄酮,可以增加 NO的水平,也是胰岛β细胞再生的诱导剂[1] [2]。
β细胞是胰岛中的一个亚群,在通过产生胰岛素调节葡萄糖的动态平衡中起重要作用。
(-)-epicatechin是胰岛β细胞再生的诱导剂。在小鼠巨噬细胞中,(-)-epicatechin抑制γ干扰素/脂多糖诱导的亚硝酸盐积累,IC50值为0.5 mM。而且,(-)-epicatechin也可以抑制0.1和1 mM过氧化亚硝酸盐(peroxynitrite)诱导的游离酪氨酸的硝化,IC50值分别为6.6和28.0 μM。在HL-60细胞中,(-)-epicatechin抑制佛波醇12-肉豆蔻酸酯13-乙酸酯(phorbol 12-myristate 13-acetate)诱导的蛋白结合酪氨酸的硝化,IC50值为10-100 μM[2]。在人内皮细胞(HUVEC)中,(-)-epicatechin (0.3-10 μM)增加NO的水平,表明其具有血管舒张活性[3]。
在四氧嘧啶(alloxan)诱导的糖尿病大鼠中,(-)-epicatechin (30 mg/kg)降低血糖,恢复胰岛中β细胞的数量,抑制四氧嘧啶诱导的β细胞坏死。在大鼠中,(-)-epicatechin在浓度高达1 g/kg时是无毒的[1]。在大鼠中,(-)-epicatechin口服给药后代谢形成3'-O-甲基-(-)-epicatechin和4'-氧-甲基-(-)-epicatechin[4]。
参考文献:
[1]. Chakravarthy BK, Gupta S, Gambhir SS, et al. Pancreatic beta-cell regeneration in rats by (-)-epicatechin. Lancet, 1981, 2(8249): 759-760.
[2]. Wippel R, Rehn M, Gorren AC, et al. Interference of the polyphenol epicatechin with the biological chemistry of nitric oxide- and peroxynitrite-mediated reactions. Biochem Pharmacol, 2004, 67(7): 1285-1295.
[3]. Brossette T, Hundsd?rfer C, Kr?ncke KD, et al. Direct evidence that (-)-epicatechin increases nitric oxide levels in human endothelial cells. Eur J Nutr, 2011, 50(7): 595-599.
[4]. Okushio K, Suzuki M, Matsumoto N, et al. Identification of (-)-epicatechin metabolites and their metabolic fate in the rat. Drug Metab Dispos, 1999, 27(2): 309-316.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 290.27 |
Cas No. | 490-46-0 |
Formula | C15H14O6 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥14.5 mg/mL in DMSO |
Chemical Name | (2R,3R)-2-(3,4-dihydroxyphenyl)chroman-3,5,7-triol |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1CC2=C(C=C(O)C=C2O)O[C@@H]1C3=CC=C(O)C(O)=C3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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