Epiallopregnanolone
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Epiallopregnanolone, a endogenous 3 β-isomer of allopregnanolone, is a metabolite of progesterone. Epiallopregnanolone is a competitive antagonist at the neurosteroid site on the GABAA receptor [1]. GABAA receptors are GABA -gated chloride channels involved in mediating neuronal inhibition in the brain [2].
In vitro: Epiallopregnanolone showed no effects on the GABA-mediated chloride flux through several types of recombinant GABA receptors. Epiallopregnanolone inhibited the allopregnanolone-stimulated GABA-mediated chloride flux through GABAA receptors. Epiallopregnanolone antagonized the inhibitory effects of allopregnanolone and ethanol on the population spike in the CA1 region of the hippocampal brain slices [1]. Epiallopregnanolone selectively blocked the allopregnanolone inhibition of the population spike in the rat hippocampal CA1[3].
In vivo: In rats trained to discriminate either 0.8g/kg or 1.2 g/kg ethanol, 100 mg/kg epiallopregnanolone treatment decreased the maximum effect by 40% and 54% ethanol lever, respectively. Epiallopregnanolone significantly decreased response rates when compared with control condition [1].
References:
[1] Ginsburg B C, Lamb R J. Alphaxalone and epiallopregnanolone in rats trained to discriminate ethanol[J]. Alcoholism: Clinical and Experimental Research, 2005, 29(9): 1621-1629.
[2] Macdonald R L, Olsen R W. GABAA receptor channels[J]. Annual review of neuroscience, 1994, 17(1): 569-602.
[3] Wang M, Bckstrm T, Landgren S. Epiallopregnanolone selectively blocks the allopregnanolone inhibition of the population spike in the rat hippocampal CA1[J]. Acta physiologica Scandinavica, 1999, 167(2): A5-A5.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 334.5 |
Cas No. | 567-01-1 |
Formula | C21H34O3 |
Synonyms | 5α-Pregnan-20-one,3β,21-dihydroxy-5α-Pregnan-20-one,3β,5α-Tetrahydrodeoxycorticosterone,3β,5α-Tetrahydroprogesterone |
Solubility | ≤25mg/ml in ethanol;25mg/ml in DMSO;25mg/ml in dimethyl formamide |
Chemical Name | 3,21-dihydroxy-, (3ß,5α)-pregnan-20-one |
SDF | Download SDF |
Canonical SMILES | C[C@@]12[C@](CC[C@@H]2C(CO)=O)([H])[C@]3([H])CC[C@@]4([H])C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
化学结构
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