Cytochalasin H
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cytochalasin H is a potent inhibitor of actin incorporation into filaments [5].
The cytochalasins are a group of cell-permeable cytotoxic fungal metabolites that disrupt actin filaments and actin-associated structures in a wide variety of cell types. Cytochalasins have been valuable agents in the study of the actin self-assembly mechanism [1][2][3][4].
Cytochalasin H is a potent inhibitor of actin incorporation into filaments. Cytochalasin H, the active constituent of an ethanolic extract of Gleditsia sinensis thorns (EEGS), had anti-angiogenic activity in vitro and in vivo via suppression of pro-angiogenic proteins, such as endothelin-1 (ET-1) and metallopeptidase 2 (MMP2). In chicken embryos, the calculated LD50 for cytochalasin H was 6.2 μg per egg [6][7].
In an in ovo tumor xenograft model, cytochalasin H significantly decreased tumor weight by 40%. In a chick CAM assay, cytochalasin H at 125 ng per egg effectively inhibited the angiogenesis by 50%. Intraperitoneal injections of cytochalasin H at 2.5 mg/kg/d inhibited A549-xenografed tumor growth without any signs of toxicity [6][7].
References:
[1]. Brenner SL, Korn ED. The effects of cytochalasins on actin polymerization and actin ATPase provide insights into the mechanism of polymerization. J Biol Chem. 1980 Feb 10;255(3):841-4.
[2]. Lin DC, Tobin KD, Grumet M, et al. Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation. J Cell Biol. 1980 Feb;84(2):455-60.
[3]. Flaumenhaft R, Dilks JR, Rozenvayn N, et al. The actin cytoskeleton differentially regulates platelet alpha-granule and dense-granule secretion. Blood. 2005 May 15;105(10):3879-87.
[4]. Walling EA1, Krafft GA, Ware BR. Actin assembly activity of cytochalasins and cytochalasin analogs assayed using fluorescence photobleaching recovery. Arch Biochem Biophys. 1988 Jul;264(1):321-32.
[5]. Yahara I, Harada F, Sekita S, et al. Correlation between effects of 24 different cytochalasins on cellular structures and cellular events and those on actin in vitro. J Cell Biol. 1982 Jan;92(1):69-78.
[6]. Lee J, Yi JM, Kim H, et al. Cytochalasin H, an active anti-angiogenic constituent of the ethanol extract of Gleditsia sinensis thorns. Biol Pharm Bull. 2014;37(1):6-12.
[7]. Yi JM, Kim J, Park JS, et al. In Vivo Anti-tumor Effects of the Ethanol Extract of Gleditsia sinensis Thorns and Its Active Constituent, Cytochalasin H. Biol Pharm Bull. 2015;38(6):909-12.
Physical Appearance | A white lyophilisate |
Storage | Store at -20°C |
M.Wt | 493.6 |
Cas No. | 53760-19-3 |
Formula | C30H39NO5 |
Synonyms | 17-Deoxo-21-acetylzygosporin D,Kodocytochalasin 1,Paspalin P1 |
Solubility | Soluble in DMSO |
Chemical Name | (3S,3aR,4S,6S,6aR,7E,10S,12R,13E,15R,15aR)-15-(acetyloxy)-2,3,3a,4,5,6,6a,9,10,11,12,15-dodecahydro-6,12-dihydroxy-4,10,12-trimethyl-5-methylene-3-1H-cycloundec[d]isoindol-1-one |
SDF | Download SDF |
Canonical SMILES | O=C1N[C@@H](CC2=CC=CC=C2)[C@]([C@]31[C@H](OC(C)=O)/C=C/[C@](C)(O)C[C@@H](C)C/C=C/[C@@]3([H])[C@@H]4O)([H])[C@H](C)C4=C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Quality Control & MSDS
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Purity ≥ 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)