Cy5 maleimide (non-sulfonated)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. The labeling reagent is a common fluorophore dye with low aqueous solubility which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. Cy5 maleimide is part of the probe among the biological research. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. The user can generate probes with virtually any non-genetically encoded moiety of interest with the scaffold in hand.
G3C(Cy5) probe consists of two parts. First, a GGGC scaffold peptide is synthesized on solid phase, followed by coupling of the liberated peptide to Cy5 maleimide, while in solution [1].
Reference:
[1]. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology,2015, 1266, 185–198.
- 1. Huan Chen, Ting Li, et al. "A nitric-oxide driven chemotactic nanomotor for enhanced immunotherapy of glioblastoma." Nat Commun. 2023 Feb 20;14(1):941. PMID: 36804924
- 2. Ting Li, Zhiyong Liu, et al. "A Universal Chemotactic Targeted Delivery Strategy for Inflammatory Diseases." Adv Mater. 2022 Nov;34(47):e2206654. PMID: 36122571
- 3. Xueting Tang, Lin Chen, et al. "Lipophilic NO-Driven Nanomotors as Drug Balloon Coating for the Treatment of Atherosclerosis." Small. 2022 Aug 12;e2203238. PMID: 35961946
- 4. Qi Wang , Ting Li, et al. "Engineered Exosomes with Independent Module/Cascading Function for Therapy of Parkinson's Disease by Multistep Targeting and Multistage Intervention Method." Adv Mater. 2022 Apr 18;e2201406. PMID: 35435282
- 5. Xiuhua Pan, Xiaochen Pei, et al. "One-in-one individual package and delivery of CRISPR/Cas9 ribonucleoprotein using apoferritin." J Control Release. 2021 Sep 10;337:686-697. PMID: 34389365
- 6. Bhatt R, Chudaev M, et al. "Engineered EF-Tu and tRNA-Based FRET Screening Assay to Find Inhibitors of Protein Synthesis in Bacteria." Assay Drug Dev Technol. 2018 May/Jun;16(4):212-221. PMID: 29870274
Physical Appearance | A solid |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
M.Wt | 641.24 |
Formula | C38H45ClN4O3 |
Solubility | ≥64.1 mg/mL in DMSO; insoluble in H2O; ≥65 mg/mL in EtOH |
Chemical Name | 6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide |
SDF | Download SDF |
Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCCN5C(=O)C=CC5=O)(C)C)C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
最大激发光波 (nm) | 646 | 最大发射光波 (nm) | 662 |
消光系数 (M-1cm-1) | 250000 | 量子产量 | 0.2 |
CF260 | 0.03 | CF280 | 0.04 |
质量控制和MSDS
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