Recombinant Human MCP-1/CCL2
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CCL2又称单核细胞趋化蛋白-1(MCP-1)和小诱导细胞因子A2，是CC趋化因子家族中的一种小细胞因子。它是一种单体多肽，分子量约为13 kDa，通过蛋白多糖的糖胺聚糖侧链锚定在内皮细胞的质膜上。研究表明，CCR2和CCR4是结合CCL2的两种细胞表面受体。CCL2对单核细胞和嗜碱性粒细胞具有趋化活性。然而，它不会吸引中性粒细胞或嗜酸性粒细胞。CCL2与银屑病、类风湿性关节炎或动脉粥样硬化等以单核细胞浸润为特征的疾病的发病机制有关，并可能参与动脉粥样硬化过程中单核细胞向动脉壁的募集。成熟的人CCL2占78%-79%的A.A.。与狗、猪和马的CCL2相同，而小鼠和大鼠表达的是一种形式的CCL2，延长了49A.A.。并且只持有约56%的股份。共同区域内的身份。
Reference
[1]. Carr MW, Roth SJ, Luther E, et al. 1994. Proc Natl Acad Sci U S A. 91:3652-6.
[2]. Yoshimura T, Yuhki N, Moore SK, et al. 1989. FEBS Lett. 244:487-93.
[3]. Furutani Y, Nomura H, Notake M, et al. 1989. Biochem Biophys Res Commun. 159:249-55.
[4]. Craig MJ, Loberg RD. 2006. Cancer Metastasis Rev. 25:611-9.
[5]. Conti P, Boucher W, Letourneau R, et al. 1995. Immunology. 86:434-40.
[6]. Bischoff SC, Krieger M, Brunner T, et al. 1992. J Exp Med. 175:1271-5.
[7]. Xia M, Sui Z. 2009. Expert Opin Ther Pat. 19:295-303.
[8]. Lloyd CM, Minto AW, Dorf ME, et al. 1997. J Exp Med. 185:1371-80.
| Gene ID | 6347 |
| Accession # | P13500 |
| Alternate Names | HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2 |
| Source | Escherichia coli. |
| M.Wt | Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence | QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles - 12 months from date of receipt, -20 to -70 °C as supplied - 1 month, 2 to 8 °C under sterile conditions after reconstitution - 3 months, -20 to -70 °C under sterile conditions after reconstitution |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuMCP-1/CCL2 as determined by LAL method.