ChIP Assay Kit (Protein A+G Agarose)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ChIP（染色质免疫沉淀）是一种用于研究蛋白质与DNA相互作用的实验技术。它允许研究者鉴定特定蛋白质（通常是转录因子或组蛋白修饰）在基因组中的结合位点。ChIP实验的基本步骤包括固定（cross-linking）、裂解、免疫沉淀、逆固定、DNA提取和DNA分析。
本产品采用了Protein A+G Agarose，适合于免疫沉淀更多种类的抗体，包括mouse IgG1，IgG2a, IgG2b, IgG3, IgA, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit IgG, rabbit and goat polyclonal Abs，以及human IgG1, IgG2, IgG3和IgG4。同时经过Salmon Sperm DNA预饱和的Protein A+G Agarose和目的基因组DNA的非特异性结合大大下降。试剂盒提供了预混合的对照引物(Control Primers)，可用于扩增human GAPDH的部分相应序列，引物序列为：5'-TACTAGCGGTTTTACGGGCG-3'；5'-TCGAACAGGAGGAGCAGAGAGCGA-3'。
ChIP Assay Kit若用于常规的染色质免疫沉淀，共可以免疫沉淀22个样品。
| Components |
K4625-22 T |
|
Protein A+G Agarose/Salmon Sperm DNA |
3 mL |
|
Glycine Solution (10X) |
30 mL |
|
ChIP Dilution Buffer |
48 mL |
|
Low Salt Immune Complex Wash Buffer |
24 mL |
|
High Salt Immune Complex Wash Buffer |
24 mL |
|
LiCl Immune Complex Wash Buffer |
24 mL |
|
TE Buffer |
48 mL |
|
0.5 M EDTA |
250 μL |
|
5 M NaCl |
500 μL |
|
1 M Tris, pH 6.5 |
500 μL |
|
SDS Lysis Buffer |
10 mL |
|
Control Primers (5 μM each) |
0.1 mL |
|
Store all the components at 4℃ for 1 year. |
|