Calpain Inhibitor II, ALLM
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Calpain inhibitor II(CPI-2)是一种细胞通透性的钙蛋白酶I、钙蛋白酶II、组织蛋白酶L和组织蛋白酶B抑制剂。
在急性淋巴细胞白血病(ALL:ALL-1、RS4;11和JURKAT)和非霍奇金淋巴瘤(NHL:RAMOS和DAUDI)细胞系中,通过MC540单荧光测量,calpain inhibitor II在50或100 μM浓度时诱导细胞凋亡。此外,研究表明,calpain inhibitor II诱导细胞凋亡不需要BTK或LYN的参与。Calpain inhibitor II对钙蛋白酶的抑制可诱导促进细胞凋亡的caspase系统[1]。与calpain inhibitor I不同,calpain inhibitor II 不能抑制NF-κB和使DLD1-TRAIL/R细胞对TRAIL蛋白敏感[2]。
参考文献:
[1] Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin's lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63.
[2] Zhu H1, Zhang L, Huang X, Davis JJ, Jacob DA, Teraishi F, Chiao P, Fang B. Overcoming acquired resistance to TRAIL by chemotherapeutic agents and calpain inhibitor I through distinct mechanisms. Mol Ther. 2004 May;9(5):666-73.
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- 2. Ednie AR, Parrish AR, et al. "Reduced hybrid/complex N-glycosylation disrupts cardiac electrical signaling and calcium handling in a model of dilated cardiomyopathy." J Mol Cell Cardiol. 2019 Jul;132:13-23. PMID: 31071333
- 3. Li W, Liu BD, et al. "Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells." Medicina (Kaunas). 2018 May 30;54(3). pii: E39. PMID: 30344270
- 4. Chen Y, Yu Y, et al. "Bradykinin promotes migration and invasion of hepatocellular carcinoma cells through TRPM7 and MMP2." Exp Cell Res. 2016 Sep 29. PMID: 27693494
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 401.57 |
Cas No. | 136632-32-1 |
Formula | C19H35N3O4S |
Synonyms | N-Acetyl-Leu-Leu-Methional |
Solubility | insoluble in H2O; ≥14.85 mg/mL in DMSO; ≥20.27 mg/mL in EtOH |
Chemical Name | 2-acetamido-4-methyl-N-[4-methyl-1-[(4-methylsulfanyl-1-oxobutan-2-yl)amino]-1-oxopentan-2-yl]pentanamide |
SDF | Download SDF |
Canonical SMILES | CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCSC)C=O)NC(=O)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
急性淋巴细胞白血病(ALL)细胞系(ALL-1、RS4;11和JURKAT)和非何杰金氏淋巴瘤(NHL)细胞系(RAMOS和DAUDI) |
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
反应条件 |
50或100 μM;24小时 |
实验结果 |
当剂量为50或100 μM时,Calpain inhibitor II诱导ALL(ALL-1、RS4;11和JURKAT)和非霍奇金淋巴瘤(RAMOS和DAUDI)细胞系凋亡。此外,研究表明,Calpain inhibitor II不需要BTK和LYN诱导细胞凋亡。Calpain inhibitor II通过抑制钙蛋白酶激活促凋亡蛋白酶系统。 |
References: [1]. Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin's lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63. |
描述 | Calpain Inhibitor II, ALLM是一种细胞通透性的钙蛋白酶I、钙蛋白酶II、组织蛋白酶L(cathepsin L)和组织蛋白酶B(cathepsin B)抑制剂,Ki值分别为120 nM、230 nM、0.6 nM和100 nM。 | |||||
靶点 | calpain I | calpain II | cathepsin L | cathepsin B | ||
IC50 | 120 nM (Ki) | 230 nM (Ki) | 0.6 nM (Ki) | 100 nM (Ki) |
质量控制和MSDS
- 批次:
化学结构
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相关生物数据
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相关生物数据
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相关生物数据
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