Bradford Protein Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Bradford法的检测原理是在酸性介质中，考马斯亮蓝G-250与蛋白质的碱性和芳香族氨基酸特别是精氨酸结合，此时溶液转变为蓝色，溶液最大吸收峰从465 nm迁移到595 nm。依据溶液颜色的变化与蛋白质浓度成正比，可通过检测595 nm处的吸光度值对溶液中蛋白质浓度进行定量测定。
Bradford Protein Assay Kit是根据Bradford法研制而成，是蛋白定量检测常用方法之一，可实现快速、稳定和高灵敏度的蛋白浓度测定，并且检测数据有很好的线性关系。
本试剂盒有如下优点：检测速度极快，和BCA法相比，10-20个样品只需不足10分钟即可完成，大大缩短了检测时间；检测灵敏度高，可实现0.5 μg蛋白量检测；标准曲线线性关系好，5 μL体积的样品或标准品在0.1-1.5 mg/mL浓度范围内有良好的线性关系。
| Components | K4103-500 T | K4103-1000 T |
| G250 reagent | 125 mL | 2 X 125 mL |
| Bovine serum albumin (BSA) | 10 mg | 20 mg |
| Bradford Protein Assay Reagent | 2 mL | 2 X 2 mL |
Store the components at 4°C for a year. | ||